Recipes

From Protists

Contents 1 Media Recipes 2 Molecular Recipes 2.1 Commonly Used 2.2 Not Used in Farmer Lab 3 Miscellaneous

Media Recipes

• AF-6 Medium
• Saltwater Medium - MSU version
• Soil Water Extract (SEX) Medium
• Artificial Seawater
• ESNW
• Hay Infusion
• ATCC 802
• CCAP S/W
• CCAP Per
• CCAP ASWP
• ATCC 1525
• HI-HS
• Chloramphenicol
• ErbsS
• Kpn Plates
• PE (Plymouth Erdshreiber)
• CH (Chalkley's Medium)
• LB-Kan plates
• RPS Media

Molecular Recipes

Commonly Used

• EDTA
• TAE
• TBE
• TE
• Tris Buffer
• Molecular Reagent Dilution Cheat Sheet
• 1M NaOH
• 5M NaCl
• 1M HCl
• 3M Sodium Acetate (NaOAc)
• DEPC Water

Not Used in Farmer Lab

• Chelex Extraction Buffer for Chelex DNA extraction protocol
• Ethidium Bromide as a substitute for GelStar to view DNA under UV light
• Loading Dye
• Lambda Buffer
• Concentration Standards with dye
• 1% Copper Acetate
• Emigs Solution

Miscellaneous

• 70% Ethanol
• Valap