Molecular Reagent Dilution Cheat Sheet
From Protists
- dNTPs - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C
| Original dNTP Mix (uL) | Autoclaved & Frozen HPLC H2O (uL) | Final Volume of Mix (uL) | Working Stock Concentration (total dNTPs) | Working Stock Concentration (each dNTP) |
|---|---|---|---|---|
| 20 | 30 | 50 | 40mM | 10mM |
| 40 | 60 | 100 | 40mM | 10mM |
| 60 | 90 | 150 | 40mM | 10mM |
| 80 | 120 | 200 | 40mM | 10mM |
- Gelstar - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container)
| Original 10,000x Gelstar (uL) | DMSO (uL) | Final Volume of Mix (uL) | Working Stock Concentration |
|---|---|---|---|
| 5 | 95 | 100 | 500x |
- Oligo Primers - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box
| Original Primer Mix (uL) | Autoclaved & Frozen HPLC H2O (uL) | Final Volume of Mix (uL) | Working Stock Concentration |
|---|---|---|---|
| 2 | 98 | 100 | 10uM |
| 4 | 196 | 200 | 10uM |
| 6 | 294 | 300 | 10uM |
- Tricolor Loading Dye - this works for the current original stock we have, which is 5x and found in the -20C (be sure to use the same buffer to dilute this as you will be using to create your gel)
| Original 5x Loading Dye (uL) | 1x TBE or TAE buffer (uL) | Final Volume of Mix (uL) | Working Stock Concentration |
|---|---|---|---|
| 100 | 400 | 500 | 1x |
| 200 | 800 | 1000 | 1x |
