ESNW

From Protists

Contents 1 ESNW Notes 2 ESNW Recipe 3 Nutrients and Trace Metals 4 ESNW Vitamins 5 See Also

ESNW Notes

Some of the cultures we have that are kept in this media include: Tetreutreptia pomquetensis (TET) Petalomonas cantuscygni (PCT)

ESNW Recipe

1. Autoclave 989mL filtered, sterilized seawater (or use 989mL of Artificial Seawater
2. Using sterile technique, add 10mL of the Nutrients and Trace Metals solution (This is brown and stored in the -20C. If stock solution is out, see Nutrients and Trace Metals subheading below on how to make it again.)
3. Using sterile technique, add 1mL of the ESNW Vitamins solution (This is pink and stored in -20C. If stock solution is out, see ESNW Vitamins subheading below on how to make it again.)

Nutrients and Trace Metals

1. Add ~700mL of distilled H2O to a LIVE media bottle
2. Add the amount specified below (g/L value) for each of the respective 10 nutrients and trace metals
3. Neutralize solution to a pH of 7.0 with HCl
4. Add distilled H2O until total volume is 1L and recheck pH to be sure it is 7.0
5. Aliquot and store in sterile tubes at -20C for future use

Nutrients and Trace Metals	g/L
NaNO3	4.667
Na2SiO3 x 9H2O	3.000
Na2glyceroPO4	0.667
Na2EDTA	0.553
H3BO3	0.380
Fe(NH4)2(SOr)2 x 6H2O	0.234
FeCl3 x 6H2O	0.016
MnSO4 x 4H2O	0.054
ZnSO4 x 7H2O	0.0073
CoSO4 x 7H2O	0.0016

ESNW Vitamins

1. Add ~700mL of distilled H2O to a LIVE media bottle
2. Add the amount specified below (g/L value) for each of the respective 3 vitamins
3. Add distilled H2O until total volume is 1L
4. Aliquot and store in sterile tubes at -20C for future use

ESNW Vitamin	g/L
Thiamine HCl	0.100
Vitamin B2	0.002
Biotin	0.001

See Also

• Recipes