Lab Knowledge Base
From Protists
Contents |
News
Wiki
- Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
- If you are new to this wiki, you will need an account in order to edit pages. Talk to Katy about getting an account set up if you would like to contribute.
- You can upload pictures, word, excel, and pdf documents.
- If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
Cultures & Lab Announcements
Next lab assistants meeting TBA
Professional Enrichment
- The Association of Women in Sciences (AWIS)
Things To Do
Priority
We are running a bit short on these medias:
- ATCC 802
- CCAP ASWP
- RPS
Instructions for making all of these are on the Wiki, please let me know if you make any of these - Katy
Next Lab Meeting TBA
October
- If you have any questions call/email Katy
- Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri
- Wipe down all molecular work benches with the FIX 70% Ethanol squirt bottle every Wed & Fri
- Check water bath on molecular work bench if low on water please add DI H2O check every Wed & Fri
- If you clean up the lab benches please email Katy, so that we know it got done
- If you're not sure what to do see Are you a Lab Assistant with free time on your hands?
List of Organisms needed by MSU
Please find these organisms and make an additional tube of them. If any are not alive or contaminated please indicate below next to the three letter codes, please email Katy with any questions
- CCM 1b: xx, alive and doing well
- EDS 2b: x, not moving
- EPE
- ETM 17a,d,f
- ELU 12j: I don't know, nothing moving.
- HYO
- KHQ
- LXC 13c: dead? contaminated?
- PET
- SXD 4i: x, alive and moving slowly
- SXE 5f: xx-xxx, alive and ok, but a few dead
- SUL 10h: x, alive, most moving
- TRT
- TUB 11e: x, but alive and really well
- TUV 3f: xxx, alive and doing well
- TUY 3b: xx, alive and doing well
- TUX 12e: xxx, alive and great
- TUM 11f: x-xx, alive and ok. Most are moving
- TUP 2g: xxx, alive and really well
- TUO 10c: xx, alive, really good
- LXB 10a: xxx, alive and great
- LXF 1g: I don't know. almost empty, maybe contaminated, maybe dead.
- LXH 1f: x, but moving a lot
- LXJ 9h: xx, alive and doing well
- LXR 6c: xx, alive, but are there 2 organisms in here?
- LXT 6e: xx, alive and doing well
- LYE 7f: xx-xxx, alive and really well, but 2 organisms?
- LYF
- LYI 2d: x-xx, alive and moving, but contaminated
- LUF 8f: xx, alive and doing well
- LUK 9a: x, alive and ok. Some are moving, some are not.
- LUT 12f: xxx, alive, doing great
- LUY 11i: xx-xxx, alive and very well, but, 2 organisms?
- TSP 10b: xx, alive and well
- Updates for Katy
- katy, i have posted the info on how to prep and ship the cultures to MSU on this page Shipping Cultures Tubes to MSU...let me know if you have any questions SEJ 08Oct09
- Euglenozoa AToL
Are you a Lab Assistant with free time on your hands?
- keep up with washing, drying and autoclaving the glass volumetric pipets so we don't run out (5, 10, 25mL)
- put cotton in the tops of pasteur pipets and fill the containers (by the dishwashing station) and autoclave them
- Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
- Autoclave media using the liquid cycle (be sure you label with autoclave tape)
- Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
- Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
- Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
- Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
- Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
- Make transfers of healthy cultures (all culture information can be found on the Cumulative Culture Inventory.xls sheet on the Farmer Lab PC desktop)
- tubes
- big/small flasks
- Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
- Extract DNA from concentrated cells and update the DNA.xls sheet
- Qualify/Quantify DNA on Nanodrop
- Maintain molecular buffers, reagents
- Maintain at least one carboy of DI water
- Keep everything clean and organized
- Ask if anyone in the lab needs anything done
Lab Binder & Notebook
- Culture Inventory
- Culture Ordering Information
- Instructional Information
- Lab Supplies
- Media and Molecular Recipes
- Protocols
- Recipes not Currently Used
Phylogenetics and Systematics of Euglenozoa
Documents
