Shipping Cultures Tubes to MSU

From Protists

Contents

Before You Get Started

  • you must allow one business day of travel (i.e. DO NOT ship on a Friday or before a federal holiday or the cultures will be sitting on a truck or in a warehouse for days and they will die)
  • make sure you have extra media made up for each of the cultures you are going to ship before you ship them
  • email Matt Bennett (bennett.m@plantbiology.msu.edu) to be sure he is ok and ready to receive the cultures or if he has a preference which day you send them (usually shipping on Monday or Tuesday is best)

Preparing Tubes and Shipping Box

  1. using sterile technique, transfer contents of culture tubes to be sent into the orange-capped 15mL Corning centrifuge tubes and fill up with appropriate media
    1. be sure each tube is labeled appropriately and clearly with the following information (this information can be looked up on the Culture Inventory excel sheet under the UGA Cultures tab by searching using the 3code):
      1. three letter code
      2. full name of organism or full ID code (ex. – Trachelomonas volvocina, Strombomonas sp., Unknown Loricate
      3. culture collection name and number (if it is from a culture collection originally; ex. UTEX 1313, CCAP 1907/2, ATCC 30904, MI 11, SAG 10.88, NJ Stromb, Ale isolate A3A11, etc.)
      4. media name
      5. date of the transfer
      6. your initials
    2. media is usually AF6, but check to be sure because some of the phototrophs are in marine or brackish water media
  2. screw caps down tightly (be sure labels are still on tubes and are legible and parafilm caps well)
  3. wrap each tube with several paper towels and scotch tape the towel to hold it in place
  4. pack the tubes carefully in a Styrofoam container to minimize the possibility of breaking tubes
  5. tape up Styrofoam container with packing tape (our tape is usually in the drawer next to the tool drawer, but tape available upstairs in CBIO office if out in our lab) and put it in cardboard box (if available) and tape that up as well


Shipping Information

  1. bring container up to CBIO office before 3pm and let them know:
    1. ship package Fedex Next Day AM
    2. ~ weight of package (if they don’t have a scale upstairs, don’t worry about it)
    3. address to ship to: Matthew Bennett, Dept. of Plant Biology, Michigan State University, S-138 Plant Biology Labs, East Lansing, MI 48824, Phone: (517) 432-0669
    4. charge it to the Farmer PEET account (ending in 278)
    5. your name, x38111 for questions if they have any
  2. email Matt to let him know shipment details (bennett.m@plantbiology.msu.edu) and can also CC the email to Mark Farmer (farmer@cb.uga.edu) and Rich Triemer (triemer@msu.edu) to keep them abreast of culture status between our labs


After Shipment

  • DO NOT toss any of our culture back-ups unless you are given the ok by both Matt and Mark (may take a couple weeks to months until Matt is sure that the cultures are growing and transferring well without contamination)
  • continue transferring culture back-ups as per normal until Matt and Mark say it is ok to toss the cultures if they are only going to be held by MSU and not UGA anymore
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