TBE
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== Background == | == Background == | ||
| - | *Tris borate with EDTA (TBE) Buffer | + | *Tris borate with EDTA (TBE) Buffer: |
**is best for electrophoresis of small (<1kb) DNA | **is best for electrophoresis of small (<1kb) DNA | ||
**provides better resolution of small (<1kb) DNA | **provides better resolution of small (<1kb) DNA | ||
**causes decreased DNA mobility | **causes decreased DNA mobility | ||
| - | **its high ionic strength and high buffering capacity means that no recirculation | + | **its high ionic strength and high buffering capacity means that no recirculation for extended run times are necessary (even in the biggest gels) |
| - | *TBE is a cheaper and easier buffer to make so we use it for nearly all of our gels that are just used to check molecular products (PCR products, gel extracts, etc.) for band size, band quantity and product quality (streaking, multiple or single bands, etc.) | + | *TBE is a cheaper and easier buffer to make (compared to [[TAE]]) so we use it for nearly all of our gels that are just used to check molecular products (PCR products, gel extracts, etc.) for band size, band quantity and product quality (streaking, multiple or single bands, etc.) |
| - | + | ||
| - | + | ||
== TBE Solutions == | == TBE Solutions == | ||
*[[10x TBE]] | *[[10x TBE]] | ||
| + | *[[5x TBE]] | ||
*[[1x TBE]] | *[[1x TBE]] | ||
| + | *[[0.5x TBE]] | ||
Current revision as of 18:07, 13 May 2008
Background
- Tris borate with EDTA (TBE) Buffer:
- is best for electrophoresis of small (<1kb) DNA
- provides better resolution of small (<1kb) DNA
- causes decreased DNA mobility
- its high ionic strength and high buffering capacity means that no recirculation for extended run times are necessary (even in the biggest gels)
- TBE is a cheaper and easier buffer to make (compared to TAE) so we use it for nearly all of our gels that are just used to check molecular products (PCR products, gel extracts, etc.) for band size, band quantity and product quality (streaking, multiple or single bands, etc.)
