5x TBE

Measure and pour ~600mL dH2O from the FIX graduated cylinder into a FIX large flask
- Graduated cylinder under sink attached to the Nanodrop/Gel counter
- Large flask in cabinets above FIX heating/stir plate
Add 3.72g Na2EDTA x 2H20 (MW 372.24; or 40mL 0.5Molar EDTA, pH 8.0 if available)
- In chemical shelves alphabetically as "EDTA disodium salt, dihydrate, crystal"
Add 27.5g Boric Acid (granular; H3BO3)
Add 54.0g Tris Base (or TRIZMA base)
Put a FIX stir bar in the large flask and cover lid with aluminum foil so dirt/dust doesn't get in mixture
Put flask on the stir plate and allow to stir until all Tris Base, Boric Acid, and Na2EDTA is dissolved
Pour mixture back into the FIX graduated cylinder and add dH2O up to 1000mL
Pour mixture back into the FIX large flask and mix well
Continue by following directions for 0.5x TBE or store in FIX 1L media bottle under sink attached to the Nanodrop/Gel counter