ESNW

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==ESNW==
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==ESNW Notes==
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Use this when cultures need brackish water.  That means it is part salt water and part [[DI water]].
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Some of the cultures we have that are kept in this media include:
Some of the cultures we have that are kept in this media include:
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''Petalomonas cantuscygni'' (PCT)
''Petalomonas cantuscygni'' (PCT)
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==View the Recipe==
 
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*Click on the link below:
 
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  http://editthis.info/images/protists/1/16/ESNW.doc
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==ESNW Recipe==
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#Autoclave '''989mL filtered, sterilized seawater''' (or use 989mL of [[Artificial Seawater]]
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#Using sterile technique, add '''10mL''' of the '''Nutrients and Trace Metals''' solution (This is brown and stored in the -20C. If stock solution is out, see Nutrients and Trace Metals subheading below on how to make it again.)
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#Using sterile technique, add '''1mL''' of the '''ESNW Vitamins''' solution (This is pink and stored in -20C.  If stock solution is out, see ESNW Vitamins subheading below on how to make it again.)
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==Nutrients and Trace Metals==
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#Add ~700mL of distilled H2O to a [[LIVE]] media bottle
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#Add the amount specified below (g/L value) for each of the respective 10 nutrients and trace metals
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#Neutralize solution to a pH of 7.0 with HCl
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#Add distilled H2O until total volume is 1L and recheck pH to be sure it is 7.0
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#Aliquot and store in sterile tubes at -20C for future use
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{| border="1" cellpadding="15" cellspacing="0"
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!Nutrients and Trace Metals
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!g/L
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|-
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|align="center"|NaNO3
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|align="center"|4.667
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|-
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|align="center"|Na2SiO3 x 9H2O
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|align="center"|3.000
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|-
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|align="center"|Na2glyceroPO4
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|align="center"|0.667
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|-
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|align="center"|Na2EDTA
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|align="center"|0.553
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|-
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|align="center"|H3BO3
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|align="center"|0.380
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|-
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|align="center"|Fe(NH4)2(SOr)2 x 6H2O
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|align="center"|0.234
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|-
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|align="center"|FeCl3 x 6H2O
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|align="center"|0.016
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|-
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|align="center"|MnSO4 x 4H2O
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|align="center"|0.054
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|-
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|align="center"|ZnSO4 x 7H2O
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|align="center"|0.0073
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|-
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|align="center"|CoSO4 x 7H2O
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|align="center"|0.0016
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|-
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|}
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==ESNW Vitamins==
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#Add ~700mL of distilled H2O to a [[LIVE]] media bottle
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#Add the amount specified below (g/L value) for each of the respective 3 vitamins
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#Add distilled H2O until total volume is 1L
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#Aliquot and store in sterile tubes at -20C for future use
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{| border="1" cellpadding="15" cellspacing="0"
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!ESNW Vitamin
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!g/L
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|-
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|align="center"|Thiamine HCl
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|align="center"|0.100
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|-
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|align="center"|Vitamin B2
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|align="center"|0.002
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|-
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|align="center"|Biotin
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|align="center"|0.001
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|-
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|}
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==See Also==
==See Also==
*[[Recipes]]
*[[Recipes]]

Current revision as of 18:23, 2 February 2009

Contents

ESNW Notes

Some of the cultures we have that are kept in this media include: Tetreutreptia pomquetensis (TET) Petalomonas cantuscygni (PCT)


ESNW Recipe

  1. Autoclave 989mL filtered, sterilized seawater (or use 989mL of Artificial Seawater
  2. Using sterile technique, add 10mL of the Nutrients and Trace Metals solution (This is brown and stored in the -20C. If stock solution is out, see Nutrients and Trace Metals subheading below on how to make it again.)
  3. Using sterile technique, add 1mL of the ESNW Vitamins solution (This is pink and stored in -20C. If stock solution is out, see ESNW Vitamins subheading below on how to make it again.)


Nutrients and Trace Metals

  1. Add ~700mL of distilled H2O to a LIVE media bottle
  2. Add the amount specified below (g/L value) for each of the respective 10 nutrients and trace metals
  3. Neutralize solution to a pH of 7.0 with HCl
  4. Add distilled H2O until total volume is 1L and recheck pH to be sure it is 7.0
  5. Aliquot and store in sterile tubes at -20C for future use


Nutrients and Trace Metals g/L
NaNO3 4.667
Na2SiO3 x 9H2O 3.000
Na2glyceroPO4 0.667
Na2EDTA 0.553
H3BO3 0.380
Fe(NH4)2(SOr)2 x 6H2O 0.234
FeCl3 x 6H2O 0.016
MnSO4 x 4H2O 0.054
ZnSO4 x 7H2O 0.0073
CoSO4 x 7H2O 0.0016


ESNW Vitamins

  1. Add ~700mL of distilled H2O to a LIVE media bottle
  2. Add the amount specified below (g/L value) for each of the respective 3 vitamins
  3. Add distilled H2O until total volume is 1L
  4. Aliquot and store in sterile tubes at -20C for future use


ESNW Vitamin g/L
Thiamine HCl 0.100
Vitamin B2 0.002
Biotin 0.001


See Also

Retrieved from "http://editthis.info/protists/ESNW"
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