ESNW
From Protists
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| - | ==ESNW== | + | ==ESNW Notes== |
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Some of the cultures we have that are kept in this media include: | Some of the cultures we have that are kept in this media include: | ||
''Tetreutreptia pomquetensis'' (TET) | ''Tetreutreptia pomquetensis'' (TET) | ||
''Petalomonas cantuscygni'' (PCT) | ''Petalomonas cantuscygni'' (PCT) | ||
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==ESNW Recipe== | ==ESNW Recipe== | ||
#Autoclave '''989mL filtered, sterilized seawater''' (or use 989mL of [[Artificial Seawater]] | #Autoclave '''989mL filtered, sterilized seawater''' (or use 989mL of [[Artificial Seawater]] | ||
| - | #Using sterile technique, add '''10mL''' of the '''Nutrients and Trace Metals''' solution (This is brown and stored in the -20C. If stock solution is out, see | + | #Using sterile technique, add '''10mL''' of the '''Nutrients and Trace Metals''' solution (This is brown and stored in the -20C. If stock solution is out, see Nutrients and Trace Metals subheading below on how to make it again.) |
| - | #Using sterile technique, add 1mL of the ESNW Vitamins solution (This is pink and stored in -20C. If stock solution is out, see | + | #Using sterile technique, add '''1mL''' of the '''ESNW Vitamins''' solution (This is pink and stored in -20C. If stock solution is out, see ESNW Vitamins subheading below on how to make it again.) |
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| + | ==Nutrients and Trace Metals== | ||
| + | |||
| + | #Add ~700mL of distilled H2O to a [[LIVE]] media bottle | ||
| + | #Add the amount specified below (g/L value) for each of the respective 10 nutrients and trace metals | ||
| + | #Neutralize solution to a pH of 7.0 with HCl | ||
| + | #Add distilled H2O until total volume is 1L and recheck pH to be sure it is 7.0 | ||
| + | #Aliquot and store in sterile tubes at -20C for future use | ||
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{| border="1" cellpadding="15" cellspacing="0" | {| border="1" cellpadding="15" cellspacing="0" | ||
!Nutrients and Trace Metals | !Nutrients and Trace Metals | ||
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| + | ==ESNW Vitamins== | ||
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| + | #Add ~700mL of distilled H2O to a [[LIVE]] media bottle | ||
| + | #Add the amount specified below (g/L value) for each of the respective 3 vitamins | ||
| + | #Add distilled H2O until total volume is 1L | ||
| + | #Aliquot and store in sterile tubes at -20C for future use | ||
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| + | |||
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{| border="1" cellpadding="15" cellspacing="0" | {| border="1" cellpadding="15" cellspacing="0" | ||
!ESNW Vitamin | !ESNW Vitamin | ||
Revision as of 18:21, 2 February 2009
Contents |
ESNW Notes
Some of the cultures we have that are kept in this media include: Tetreutreptia pomquetensis (TET) Petalomonas cantuscygni (PCT)
ESNW Recipe
- Autoclave 989mL filtered, sterilized seawater (or use 989mL of Artificial Seawater
- Using sterile technique, add 10mL of the Nutrients and Trace Metals solution (This is brown and stored in the -20C. If stock solution is out, see Nutrients and Trace Metals subheading below on how to make it again.)
- Using sterile technique, add 1mL of the ESNW Vitamins solution (This is pink and stored in -20C. If stock solution is out, see ESNW Vitamins subheading below on how to make it again.)
Nutrients and Trace Metals
- Add ~700mL of distilled H2O to a LIVE media bottle
- Add the amount specified below (g/L value) for each of the respective 10 nutrients and trace metals
- Neutralize solution to a pH of 7.0 with HCl
- Add distilled H2O until total volume is 1L and recheck pH to be sure it is 7.0
- Aliquot and store in sterile tubes at -20C for future use
| Nutrients and Trace Metals | g/L |
|---|---|
| NaNO3 | 4.667 |
| Na2SiO3 x 9H2O | 3.000 |
| Na2glyceroPO4 | 0.667 |
| Na2EDTA | 0.553 |
| H3BO3 | 0.380 |
| Fe(NH4)2(SOr)2 x 6H2O | 0.234 |
| FeCl3 x 6H2O | 0.016 |
| MnSO4 x 4H2O | 0.054 |
| ZnSO4 x 7H2O | 0.0073 |
| CoSO4 x 7H2O | 0.0016 |
ESNW Vitamins
- Add ~700mL of distilled H2O to a LIVE media bottle
- Add the amount specified below (g/L value) for each of the respective 3 vitamins
- Add distilled H2O until total volume is 1L
- Aliquot and store in sterile tubes at -20C for future use
| ESNW Vitamin | g/L |
|---|---|
| Thiamine HCl | 0.100 |
| Vitamin B2 | 0.002 |
| Biotin | 0.001 |
