Inoculate with bacteria

From Protists

• To inoculate a media with bacteria, start with a flask of the desired media made under the hood.
• Open the flask and flame the opening.
• Grab the plate of desired bacteria out of the 4C walk-in fridge. (Wear gloves if desired)
• Under the hood, there is an inoculating loop in a graduated cylinder containing ethanol. Flame the loop until it glows orange. Continue flaming down the wire until you reach the handle.
• Allow the loop to cool for ~5 secs or cool the loop by touching it to the agar of your plate in an area that has no bacteria growing.
• When the loop is cool, swipe a colony or a strip of bacteria with the loop. (Only open the bacterial plate long enough to swipe a colony and then immediately replace it upside-down onto the plate cover)
• Put the loop in the media in your flask and shake it around.
• Take the loop out and flame it again as previously described.
• At this point, you can either repeat the inoculation process with another type of bacteria in the same flask or inoculate a second flask with bacteria. (Always flame the loop between inoculation steps)
• When done inoculating, flame the loop one more time.
• Allow it to cool for 5 secs and put the loop end down into the graduated cylinder containing ethanol under the hood.
• Flame the opening of your flask and close it.
• Grow the media with bacteria in it in incubator 5B (middle shelf) at 34C. Replace the bacterial plate in its position in the 4C walk-in fridge with the agar side up.
• The media should be ready to for inoculation by a protist the next day.