Molecular Reagent Dilution Cheat Sheet

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  • dNTPs - this works for the current original stock we have 100mM total dNTP concentration (25mM/dNTP)
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Original dNTP Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix Final Mix Concentration (total dNTPs) Final Mix Concentration (each dNTP)
20 30 50 40 10
40 60 100 40 10
60 90 150 40 10
80 120 200 40 10
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