10x TE

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Revision as of 15:40, 1 October 2007 by Admin (Talk | contribs)
  • add 1.219g Tris to a FIX 100mL graduated cylinder
  • add 0.37g EDTA
  • add up to 100mL dH2O and mix well (cover with parafilm to prevent leakage)
  • check and adjust pH to 8.0 if necessary
  • store in small FIX media bottle under sink attached to the Nanodrop/Gel counter

Dilute 10x TE to working stock 1x TE prior to its use in molecular work.

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