Lab Knowledge Base

Revision as of 03:38, 22 August 2007 by Admin (Talk | contribs)

News

Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
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Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
Autoclave media using the liquid cycle (be sure you label with autoclave tape)
Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip boxes, volumetric pipets, etc. in appropriate containers with containers underneath if available
Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
Make transfers of healthy cultures
- tubes
- big/small flasks
Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
Extract DNA from concentrated cells
Qualify/Quantify DNA on Nanodrop
Maintain molecular buffers, reagents
Maintain at least one carboy of DI water
Keep everything clean and organized
Ask if anyone in the lab needs anything done