Lab Knowledge Base

From Protists

Revision as of 17:14, 4 September 2009

News

Wiki

• Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
• If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
• You can upload pictures, word, excel, and pdf documents.
• If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.

Cultures & Lab Announcements

Next lab assistants meeting TBA

Recap of lab assistants meeting Wednesday, September 2nd at 3:30 pm

• Organisms to send to MSU
• Organisms with contamination: PLO
• Update inventory of chemicals for MSDS binder
• Updates to the Wiki

Professional Enrichment

• The Association of Women in Sciences (AWIS)

Things To Do

Priority

See the list of organisms needed by MSU below

Next Lab Meeting TBA

Week of Aug 31 - Sept 4

• If you have any questions call/email Katy
• Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri wed done TA/DO 2sept
• Wipe down all molecular work benches with the FIX 70% Ethanol squirt bottle every Wed & Fri wed done TA/DO 2sept
• The wiki is in the updating process if you're not sure what to do see Are you a Lab Assistant with free time on your hands?

Lets find out what happened to these cultures

Please email Katy if you find these cultures with the status and location Done AI 4sep09 Caitlin- here are the organisms you set out for me to transfer to small flask today (12 Jun 09)

TXG-->13Nov08-->XX

UEA-->13Nov08-->X

SUB-->NODATE-->XXXX

MPI-->17APR09-->X

TUH-->6MAR09-->X

List of Organisms needed by MSU

Please find these organisms and make an additional tube of them. If any are not alive or contaminated please indicate below next to the three letter codes, please email Katy with any questions

• CCM 1b • EDS 2b • EPE • ETM 17a,d,f • ELU 12j • HYO • KHQ • LXC 13c • PET • SXD 4i • SXE 5f • SUL 10h • TRT • TUB 11e • TUV 3f • TUY 3b • TUX 12e • TUM 11f • TUP 2g • TUO 10c • LXB 10a • LXF 1g • LXH 1f • LXJ 9h • LXR 6c • LXT 6e • LYE 7f • LYF • LYI 2d • LUF 8f • LUK 9a • LUT 12f • LUY 11i • TSP 10b

• Updates for Katy

Notes for Sarah

• Euglenozoa AToL

Are you a Lab Assistant with free time on your hands?

• keep up with washing, drying and autoclaving the glass volumetric pipets so we don't run out (5, 10, 25mL)
• put cotton in the tops of pasteur pipets and fill the containers (by the dishwashing station) and autoclave them
• Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
• Autoclave media using the liquid cycle (be sure you label with autoclave tape)
• Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
• Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
• Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
• Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
• Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
• Make transfers of healthy cultures (all culture information can be found on the Cumulative Culture Inventory.xls sheet on the Farmer Lab PC desktop)
  • tubes
  • big/small flasks
• Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
• Extract DNA from concentrated cells and update the DNA.xls sheet
• Qualify/Quantify DNA on Nanodrop
• Maintain molecular buffers, reagents
• Maintain at least one carboy of DI water
• Keep everything clean and organized
• Ask if anyone in the lab needs anything done

Lab Binder & Notebook

• Culture Inventory
• Culture Ordering Information
• Instructional Information
• Lab Supplies
• Media and Molecular Recipes
• Protocols
• Recipes not Currently Used

Phylogenetics and Systematics of Euglenozoa

• Euglenozoa Phylogenies
• Euglenophyte Phylogenies
• Phylogenetic Programs
• Farmer Lab

Documents

• PCR Reactions
• DNA Dilution Quick Calculation

See Also

• http://meta.wikimedia.org/wiki/Help:Wikitext_examples