Lab Knowledge Base

From Protists

Revision as of 19:03, 25 March 2009

News

Wiki

• Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
• If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
• You can upload pictures, word, excel, and pdf documents.
• If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.

Cultures & Lab Announcements

Professional Enrichment

• The Association of Women in Sciences (AWIS)

Pressing Things TO DO!

Next Lab Meeting (27Mar09 @ 2:30pm)

1. Updates on Sarah's culture stuff (i.e. Large flask status, backup tube status, incubator 2 stuff, backup backup racks)
2. Go over using refractometer

Today (25Mar09)

Things that must be done today or tomorrow:

• call SEJ or CC if you have any questions (if not at lab, then working from home)

• Check large flasks of RBS and PLO for spin downs/DNA extraction (checked by CC - See instruction below)
  • PLO (6C) - Not ready to be spun down yet (3/25/09)
  • RBS (6A) - Not ready to be spun down yet (3/25/09)
• CS - Start list on Wiki of Sarah's large flasks and their locations.
• JI & AI - Please check to see if all of the large flasks have backup tubes. If not, please start backup tubes for them. See Ben's list of large flasks to find all of them. Start a new rack if necessary.
  • DO has checked to see if we have backup test tubes for all flasks in incubator 3 and 4
  • Check DO's notes in the notebook to see if we need to make any test tubes from flasks
• check flasks and spin down if ready... (don't forget the flasks in the #2 and #3 incubators)...switch over any of Sarah's in closed flasks into vented flasks or start new small ones from scratch if original big flasks dead
• DO - check sarah's big and small flasks of incubator 3 (please note specifically everything you did in the notebook)
  • spin down any large or small flasks that are in log phase spun down small flasks DO
  • refresh any flasks that are in stationary phase done DO
  • make new small flasks from their original test tube if there are any dead large or small flasks LVO was the only dead flask; threw out - has a backup test tube - DO
  • change any plug sealed flasks to ventilated flasks done DO
• Check all colorless organisms except: PER, PLO, and RBS done CS&BD 24Mar09 - will refresh 25Mar09
• CS&BD - work on incubator 2

If not finished, the last person to leave should e-mail SEJ or CC what's left to be done (depending on who it's for).

Priority:

• gDNA extraction of SNB (see Sarah and CC to see if more need to be done too)...will need RBS, DIP and PLO gDNA as well
• update UGA flasks sheet as to what we have in flasks...there were a bunch of small flasks at the bottom of incubator 4 that were completely dried down and never transferred to big flasks, so SEJ tossed these and they shouldn't be on the list now unless there are big flasks for them
• Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri
• Wipe down all molecular work benches with the FIX 70% Ethanol squirt bottle every Wed & Fri

Cultures:

Lab Assistants, Please Update Sarah/Christina On:

• Updates for Sarah
  • list of all cultures I need in small flasks that have been started (any moved to big flasks yet?)
  • list of all cultures I need in small flasks that are contaminated (and preferably what they are contaminated with)

• Updates for Christina

Notes for Sarah

• Euglenozoa AToL

• • Can Joy or someone find out where the EGN and LVO tubes are and make small flasks from them, the small flasks are already labeled and are located near the saltwater carboys, change the initials to fit yourself please (note from DO!) done JI 5Mar09

Are you a Lab Assistant with free time on your hands?

• keep up with washing, drying and autoclaving the glass volumetric pipets so we don't run out (5, 10, 25mL)
• put cotton in the tops of pasteur pipets and fill the containers (by the dishwashing station) and autoclave them
• Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
• Autoclave media using the liquid cycle (be sure you label with autoclave tape)
• Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
• Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
• Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
• Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
• Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
• Make transfers of healthy cultures (all culture information can be found on the Cumulative Culture Inventory.xls sheet on the Farmer Lab PC desktop)
  • tubes
  • big/small flasks
• Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
• Extract DNA from concentrated cells and update the DNA.xls sheet
• Qualify/Quantify DNA on Nanodrop
• Maintain molecular buffers, reagents
• Maintain at least one carboy of DI water
• Keep everything clean and organized
• Ask if anyone in the lab needs anything done

Lab Binder & Notebook

• Protocols
• Media and Molecular Recipes
• Culture Inventory
• Inventory
• Instructional Information
• Recipes not Currently Used
• Culture Ordering Information

Phylogenetics and Systematics of Euglenozoa

• Euglenozoa Phylogenies
• Euglenophyte Phylogenies
• Phylogenetic Programs
• Farmer Lab

Documents

• PCR Reactions
• DNA Dilution Quick Calculation

See Also

• http://meta.wikimedia.org/wiki/Help:Wikitext_examples