RPS Media
From Protists
(Difference between revisions)
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*Make the following solution: | *Make the following solution: | ||
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|Biotin | |Biotin | ||
|0.125g | |0.125g | ||
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*Make the following solution: | *Make the following solution: | ||
| - | {| | + | {| border="1" cellpadding="10" cellspacing="0" |
|align="center"|Metal | |align="center"|Metal | ||
|align="center"|f/2 metal solution conc | |align="center"|f/2 metal solution conc | ||
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*Filter 1L from the top of the 1X Ultramarine carboy using Whatman #1 filters. | *Filter 1L from the top of the 1X Ultramarine carboy using Whatman #1 filters. | ||
*Pour 998 mL into a clean, autoclaved media bottle. | *Pour 998 mL into a clean, autoclaved media bottle. | ||
| - | *Under the hood, add 1mL of the RPS Metal Working Stock and 1mL of the RPSv (Vitamin Stock). | + | *Under the flame in the hood, add 1mL of the RPS Metal Working Stock and 1mL of the RPSv (Vitamin Stock). |
*Mix thoroughly. | *Mix thoroughly. | ||
*Label "RPS Media" and autoclave. | *Label "RPS Media" and autoclave. | ||
*Store in Media Cabinet. | *Store in Media Cabinet. | ||
Current revision as of 23:02, 10 March 2009
RPS Vitamin Solution
- Make the following solution:
| Biotin | 0.125g |
| Vit B12 | 0.125g |
| Thiamine HCl | 0.025g |
| dH2O | 250ml |
Biotin and Vit B12 in drawer in 4C. Thiamine HCl in -20C in door.
- Mix thoroughly.
- Aliquot 10mL into 1mL aliquots in microcentrifuge tubes.
- Label all tubes with "RPSv" for RPS vitamin stock.
- Store at -20C in "Tina's Box".
- Label the rest of the original bottle "RPS Vitamin Stock" with the date and your initials.
- Store the rest of the stock at -20C in the door.
RPS Metal Working Stock
- Make the following solution:
| Metal | f/2 metal solution conc | Amount |
| Na2EDTA.2H2O | 2.180g/500mL | 500uL |
| FeCl3.6H2O | 1.575g/500mL | 500uL |
| CuSO4.5H2O | 9.8g/L | 500uL |
| ZnSO4.7H2O | 22g/L | 500uL |
| CoCl2.6H2O | 10g/L | 500uL |
| MnCl2.4H2O | 180g/L | 50uL |
| NaMoO4.2H2O | 12.6g/L | 500uL |
| dH2O | -- | to 500mL total volume |
All f/2 metal stocks in 4C walk-in.
- Mix thoroughly.
- Label "RPS Metal Working Stock".
- Store in 4C walk-in.
RPS Media
- Make Art. Seawater using 33g of Ultramarine Sea Salts and 1L of dH20.
- Heat to boiling while stirring. (use hot plate)
- Pour into 1X Ultramarine carboy and let settle for 2 days.
- Filter 1L from the top of the 1X Ultramarine carboy using Whatman #1 filters.
- Pour 998 mL into a clean, autoclaved media bottle.
- Under the flame in the hood, add 1mL of the RPS Metal Working Stock and 1mL of the RPSv (Vitamin Stock).
- Mix thoroughly.
- Label "RPS Media" and autoclave.
- Store in Media Cabinet.
