CCAP ASWP
From Protists
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= CCAP ASWP Stock Solution = | = CCAP ASWP Stock Solution = | ||
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*Aliquot into smaller vials (15mL tubes) and put in -20C for long term storage. | *Aliquot into smaller vials (15mL tubes) and put in -20C for long term storage. | ||
*Thaw when necessary in warm water. | *Thaw when necessary in warm water. | ||
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= CCAP Soil Extract Soln = | = CCAP Soil Extract Soln = | ||
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# Adjust pH to 7.0-8.0 with 1M HCl ot 1M NaOH, if necessary. (Typically slightly acidic) | # Adjust pH to 7.0-8.0 with 1M HCl ot 1M NaOH, if necessary. (Typically slightly acidic) | ||
# Store in Media Cabinet as "CCAP Soil Extract Soln". | # Store in Media Cabinet as "CCAP Soil Extract Soln". | ||
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= CCAP ASWP Medium = | = CCAP ASWP Medium = | ||
Revision as of 20:38, 7 November 2007
CCAP ASWP Stock Solution
Mix the following ingredients:
| NaNO3 | 0.563g | |
| NaHPO4.7H2O | 0.043g | |
| K2HPO4 | 0.019g | |
| Distilled H2O | 100mL |
- Label as "CCAP ASWP Stock Soln".
- Aliquot into smaller vials (15mL tubes) and put in -20C for long term storage.
- Thaw when necessary in warm water.
CCAP Soil Extract Soln
- Put 105g of soil and 660 mL of deionized water into a 1L-bottle.
- Autoclave for 15 minutes at 15 psi on 2 consecutive days.
- Let contents settle for ~7 days.
- Decant and filter the supernatant liquid.
- Adjust pH to 7.0-8.0 with 1M HCl ot 1M NaOH, if necessary. (Typically slightly acidic)
- Store in Media Cabinet as "CCAP Soil Extract Soln".
CCAP ASWP Medium
Mix the following ingredients:
| Ultramarine Synthetica Sea Salts | 8.4g | |
| Tricine | 0.125g | |
| CCAP Stock Soln | 2.5mL | |
| CCAP Soil Extract Soln | 12.5mL | |
| Deionized H2O | 235mL |
- Total volume should be 250 mL.
- Adjust pH to 7.6-7.8 with 1M Hcl or 1M NaOH, as necessary.
- Label as "CCAP ASWP".
- Autoclave at 15 psi for 15 minutes.
- Store in Media Cabinet.
