Molecular Reagent Dilution Cheat Sheet

(Difference between revisions)

Revision as of 14:10, 16 October 2007

dNTPs - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C

Original dNTP Mix (uL)	Autoclaved & Frozen HPLC H₂O (uL)	Final Volume of Mix (uL)	Working Stock Concentration (total dNTPs)	Working Stock Concentration (each dNTP)
20	30	50	40mM	10mM
40	60	100	40mM	10mM
60	90	150	40mM	10mM
80	120	200	40mM	10mM

Gelstar - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container)

Original 10,000x Gelstar (uL)	DMSO (uL)	Final Volume of Mix (uL)	Working Stock Concentration
5	95	100	500x

Oligo Primers - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box

Original Primer Mix (uL)	Autoclaved & Frozen HPLC H₂O (uL)	Final Volume of Mix (uL)	Working Stock (total dNTPs)
2	98	100	10uM
4	196	200	10uM
6	294	300	10uM

@@ Line 4: / Line 4: @@
 ! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
 ! Final Volume of Mix (uL)
-! Final Mix Concentration (total dNTPs)
+! Working Stock Concentration (total dNTPs)
-! Final Mix Concentration (each dNTP)
+! Working Stock Concentration (each dNTP)
 |-
 | 20
@@ Line 40: / Line 40: @@
 ! DMSO (uL)
 ! Final Volume of Mix (uL)
-! Final Mix Concentration
+! Working Stock Concentration
 |-
 | 5
@@ Line 56: / Line 56: @@
 ! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
 ! Final Volume of Mix (uL)
-! Final Mix Concentration (total dNTPs)
+! Working Stock (total dNTPs)
 |-
 | 2