TBE

From Protists

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Revision as of 14:54, 1 October 2007

Background

• Tris borate with EDTA (TBE) Buffer
  • is best for electrophoresis of small (<1kb) DNA
  • provides better resolution of small (<1kb) DNA
  • causes decreased DNA mobility
  • its high ionic strength and high buffering capacity means that no recirculation or extended run times are necessary
• TBE is a cheaper and easier buffer to make so we use it for nearly all of our gels that are just used to check molecular products (PCR products, gel extracts, etc.) for band size, band quantity and product quality (streaking, multiple or single bands, etc.)

TBE Solutions

• 10x TBE
• 1x TBE