Lab Knowledge Base

(Difference between revisions)

Revision as of 15:39, 6 September 2007

Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
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Informational Seminar in Research:
- When: 12pm (noon), September 12th
- Where: Room H236 at the College of Veterinary Medicine
- Who: all students are welcome to hear Dr. Khanna, DVM, PhD, Dip. ACVIM (oncology) and Dr. Melissa Paoloni, DVM, Dip. ACVIM (oncology)
- What: The Drs. will speak with students about a career in research, what is NIH, what is NCI, what it is like to work at NIH, opportunities at NIH, and how to get started in the research field.

LEARN HOW TO DO DNA Extraction Protocol
tell SEJ about any supplies that we are out of/running low on so she can purchase them
- yellow pipet tips
- microcentrifuge tubes with graduations
- other stuff?
transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)

Last Friday/Saturday? - Caitlin and Joy...This week, finish up what hasn't been done already.

check big flasks & spin down if necessary
check back up small flasks & "no cells" small flasks in the culture room 20C incubator
check on ENTO, PER, Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
make transfers of the best ENTO, PER, TET and if Eutreptia pertyi and Eutreptiella sp. are ready, transfer them too
how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary

Other:

update culture inventory with MSU cultures and the 3letter codes SEJ gave them
wash dishes (make sure dry before putting away!)
set up new answering machine to lab phone
clean centrifuge rotors (read manuals for how to do this...5417C and the one next to it from the Keith lab)
hang up SEJ's poster (& Sheila's when she brings it in)

Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
Autoclave media using the liquid cycle (be sure you label with autoclave tape)
Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
Make transfers of healthy cultures
- tubes
- big/small flasks
Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
Extract DNA from concentrated cells
Qualify/Quantify DNA on Nanodrop
Maintain molecular buffers, reagents
Maintain at least one carboy of DI water
Keep everything clean and organized
Ask if anyone in the lab needs anything done

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 *You can upload pictures, word, excel, and pdf documents.
 *If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
-*'''WELCOME BACK STUDENTS AND GOOD LUCK THIS FALL SEMESTER!!!'''
+*'''Informational Seminar in Research:'''
+**'''When:''' 12pm (noon), September 12th
+**'''Where:''' Room H236 at the College of Veterinary Medicine
+**'''Who:''' all students are welcome to hear [http://ccr.ncifcrf.gov/Staff/staff.asp?profileid=8295  Dr. Khanna], DVM, PhD, Dip. ACVIM (oncology) and Dr. Melissa Paoloni, DVM, Dip. ACVIM (oncology)
+**'''What:''' The Drs. will speak with students about a career in research, what is NIH, what is NCI, what it is like to work at NIH, opportunities at NIH, and how to get started in the research field.