Lab Knowledge Base
From Protists
(Difference between revisions)
(→Pressing Things TO DO!) |
(→Pressing Things TO DO!) |
||
| Line 11: | Line 11: | ||
*transfer all back up test tube racks! (already started so check in notebook to see where the last person left off) | *transfer all back up test tube racks! (already started so check in notebook to see where the last person left off) | ||
*check on ENTO, PER, ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!) | *check on ENTO, PER, ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!) | ||
| - | * | + | |
| - | * | + | |
| + | '''THURSDAY - Stephen and Joy''' | ||
| + | *Caitlin already weighed out all of the chemicals you will need for the AF6 stock chemicals...check where she left off (should add 200mL + pre-weighed chemicals to appropriate stock bottle and then shake/mix until all chemical particles dissolved) | ||
| + | *finish making AF6 stock chemicals | ||
| + | *make up lots of AF6 & autoclave it | ||
| + | *keep checking test tube racks (can work ahead) and then transfer them when new AF6 cooled enough | ||
| + | *check big flasks & spin down if necessary | ||
| + | *check back up small flasks & "no cells" small flasks in the culture room 20C incubator | ||
| + | |||
| + | |||
| + | '''Other:''' | ||
| + | *update culture inventory with MSU cultures and the 3letter codes SEJ gave them | ||
| + | *wash dishes | ||
| + | *move empty boxes under black table in culture room and break down | ||
| + | *move everything off of black table (carboys can go under big black table in the transfer hood room) | ||
| + | *move small black table into the transfer hood room (end to end with the other big black table)...will need at least two people b/c have to take off black top (w/ power drill), move base and top separately into transfer hood room and then reassemble the table | ||
== Are you a Lab Assistant with free time on your hands? == | == Are you a Lab Assistant with free time on your hands? == | ||
Revision as of 14:26, 30 August 2007
Contents |
News
- Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
- If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
- You can upload pictures, word, excel, and pdf documents.
- If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
- WELCOME BACK STUDENTS AND GOOD LUCK THIS FALL SEMESTER!!!
Pressing Things TO DO!
- tell SEJ about any supplies that we are out of/running low on so she can purchase them
- transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)
- check on ENTO, PER, Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
THURSDAY - Stephen and Joy
- Caitlin already weighed out all of the chemicals you will need for the AF6 stock chemicals...check where she left off (should add 200mL + pre-weighed chemicals to appropriate stock bottle and then shake/mix until all chemical particles dissolved)
- finish making AF6 stock chemicals
- make up lots of AF6 & autoclave it
- keep checking test tube racks (can work ahead) and then transfer them when new AF6 cooled enough
- check big flasks & spin down if necessary
- check back up small flasks & "no cells" small flasks in the culture room 20C incubator
Other:
- update culture inventory with MSU cultures and the 3letter codes SEJ gave them
- wash dishes
- move empty boxes under black table in culture room and break down
- move everything off of black table (carboys can go under big black table in the transfer hood room)
- move small black table into the transfer hood room (end to end with the other big black table)...will need at least two people b/c have to take off black top (w/ power drill), move base and top separately into transfer hood room and then reassemble the table
Are you a Lab Assistant with free time on your hands?
- Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
- Autoclave media using the liquid cycle (be sure you label with autoclave tape)
- Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
- Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
- Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
- Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
- Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
- Make transfers of healthy cultures
- tubes
- big/small flasks
- Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
- Extract DNA from concentrated cells
- Qualify/Quantify DNA on Nanodrop
- Maintain molecular buffers, reagents
- Maintain at least one carboy of DI water
- Keep everything clean and organized
- Ask if anyone in the lab needs anything done
Lab Binder & Notebook
- Protocols
- Media Recipes
- Inventory
- Instructional Information
- Recipes not Currently Used
- Culture Ordering Information
Phylogenetics and Systematics of Euglenozoa
Documents
