Molecular Reagent Dilution Cheat Sheet

From Protists

(Difference between revisions)
Line 46: Line 46:
| 100
| 100
| 500x
| 500x
 +
|-
 +
|}
 +
 +
 +
 +
*[[Oligo Primers]] - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
! Original Primer Mix (uL)
 +
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
 +
! Final Volume of Mix (uL)
 +
! Final Mix Concentration (total dNTPs)
 +
|-
 +
| 2
 +
| 98
 +
| 100
 +
| 10uM
 +
|-
 +
| 4
 +
| 196
 +
| 200
 +
| 10uM
 +
|-
 +
| 6
 +
| 294
 +
| 300
 +
| 10uM
|-
|-
|}
|}

Revision as of 14:09, 16 October 2007

  • dNTPs - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C
Original dNTP Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix (uL) Final Mix Concentration (total dNTPs) Final Mix Concentration (each dNTP)
20 30 50 40mM 10mM
40 60 100 40mM 10mM
60 90 150 40mM 10mM
80 120 200 40mM 10mM


  • Gelstar - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container)
Original 10,000x Gelstar (uL) DMSO (uL) Final Volume of Mix (uL) Final Mix Concentration
5 95 100 500x


  • Oligo Primers - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box
Original Primer Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix (uL) Final Mix Concentration (total dNTPs)
2 98 100 10uM
4 196 200 10uM
6 294 300 10uM
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