Molecular Reagent Dilution Cheat Sheet
From Protists
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | *[[dNTPs]] - this works for the current original stock we have 100mM total dNTP concentration (25mM/dNTP) | + | *[[dNTPs]] - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C |
{| border="1" cellspacing="0" cellpadding="5" align="center" | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
! Original dNTP Mix (uL) | ! Original dNTP Mix (uL) | ||
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL) | ! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL) | ||
| - | ! Final Volume of Mix | + | ! Final Volume of Mix (uL) |
! Final Mix Concentration (total dNTPs) | ! Final Mix Concentration (total dNTPs) | ||
! Final Mix Concentration (each dNTP) | ! Final Mix Concentration (each dNTP) | ||
| Line 30: | Line 30: | ||
| 40mM | | 40mM | ||
| 10mM | | 10mM | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | *[[Gelstar]] - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container) | ||
| + | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
| + | ! Original 10,000x Gelstar (uL) | ||
| + | ! DMSO (uL) | ||
| + | ! Final Volume of Mix (uL) | ||
| + | ! Final Mix Concentration | ||
| + | |- | ||
| + | | 5 | ||
| + | | 95 | ||
| + | | 100 | ||
| + | | 500x | ||
|- | |- | ||
|} | |} | ||
Revision as of 14:04, 16 October 2007
- dNTPs - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C
| Original dNTP Mix (uL) | Autoclaved & Frozen HPLC H2O (uL) | Final Volume of Mix (uL) | Final Mix Concentration (total dNTPs) | Final Mix Concentration (each dNTP) |
|---|---|---|---|---|
| 20 | 30 | 50 | 40mM | 10mM |
| 40 | 60 | 100 | 40mM | 10mM |
| 60 | 90 | 150 | 40mM | 10mM |
| 80 | 120 | 200 | 40mM | 10mM |
- Gelstar - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container)
| Original 10,000x Gelstar (uL) | DMSO (uL) | Final Volume of Mix (uL) | Final Mix Concentration |
|---|---|---|---|
| 5 | 95 | 100 | 500x |
