Molecular Reagent Dilution Cheat Sheet

From Protists

(Difference between revisions)
Line 1: Line 1:
*[[dNTPs]] - this works for the current original stock we have 100mM total dNTP concentration (25mM/dNTP)
*[[dNTPs]] - this works for the current original stock we have 100mM total dNTP concentration (25mM/dNTP)
-
{| border="1" cellspacing="0"  
+
{| border="1" cellspacing="0" cellpadding="5" align="center"
-
cellpadding="5" align="center"
+
! Original dNTP Mix (uL)
! Original dNTP Mix (uL)
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
Line 11: Line 10:
| 30
| 30
| 50
| 50
-
| 40
+
| 40mM
-
| 10
+
| 10mM
|-
|-
| 40
| 40
| 60
| 60
| 100
| 100
-
| 40
+
| 40mM
-
| 10
+
| 10mM
|-
|-
| 60
| 60
| 90
| 90
| 150
| 150
-
| 40
+
| 40mM
-
| 10
+
| 10mM
|-
|-
| 80
| 80
| 120
| 120
| 200
| 200
-
| 40
+
| 40mM
-
| 10
+
| 10mM
|-
|-
|}
|}

Revision as of 13:59, 16 October 2007

  • dNTPs - this works for the current original stock we have 100mM total dNTP concentration (25mM/dNTP)
Original dNTP Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix Final Mix Concentration (total dNTPs) Final Mix Concentration (each dNTP)
20 30 50 40mM 10mM
40 60 100 40mM 10mM
60 90 150 40mM 10mM
80 120 200 40mM 10mM
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