Lab Knowledge Base

(Difference between revisions)

Revision as of 18:03, 28 August 2007

Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
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WELCOME BACK STUDENTS AND GOOD LUCK THIS FALL SEMESTER!!!

tell SEJ about any supplies that we are out of/running low on so she can purchase them
transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)
check on ENTO, PER, Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
scan in two documents for MAF (for small one put black sheet behind and scan at low res for text pages and higher for pic pages)
break down cardboard boxes by dry erase board and put under overhang under air conditioner (or with other flattened boxes) out on the loading dock-recycling area

Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
Autoclave media using the liquid cycle (be sure you label with autoclave tape)
Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
Make transfers of healthy cultures
- tubes
- big/small flasks
Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
Extract DNA from concentrated cells
Qualify/Quantify DNA on Nanodrop
Maintain molecular buffers, reagents
Maintain at least one carboy of DI water
Keep everything clean and organized
Ask if anyone in the lab needs anything done

@@ Line 12: / Line 12: @@
 *check on ENTO, PER, ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!)
 *scan in two documents for MAF (for small one put black sheet behind and scan at low res for text pages and higher for pic pages)
+*break down cardboard boxes by dry erase board and put under overhang under air conditioner (or with other flattened boxes) out on the loading dock-recycling area
 == Are you a Lab Assistant with free time on your hands? ==