Lab Knowledge Base

From Protists

(Difference between revisions)
(→Cultures & Lab Announcements)
(→Pressing Things TO DO!)
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== Pressing Things TO DO! ==  
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== Things To Do ==  
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=== Next Lab Meeting (September 2, 2009 @ 3:30pm) ===
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=== Priority ===
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See the list of organisms needed by MSU below
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#Meet new student, Talal
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=== Next Lab Meeting TBA ===
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#Update the Wiki
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#Check out new [[Lab Supplies]] page
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#Updates on cultures and spindowns
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*If you have any questions call/email Katy
*If you have any questions call/email Katy
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*Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri '''wed done TA 2sept'''
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*Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri '''wed done TA/DO 2sept'''
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*Wipe down all molecular work benches with the [[FIX]] 70% Ethanol squirt bottle every Wed & Fri
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*Wipe down all molecular work benches with the [[FIX]] 70% Ethanol squirt bottle every Wed & Fri '''wed done TA/DO 2sept'''
*The wiki is in the updating process if you're not sure what to do see '''Are you a Lab Assistant with free time on your hands?'''  
*The wiki is in the updating process if you're not sure what to do see '''Are you a Lab Assistant with free time on your hands?'''  
 +
=== Lets find out what happened to these cultures ===
 +
Please email Katy if you find these cultures with the status and location
 +
'''Caitlin- here are the organisms you set out for me to transfer to small flask today (12 Jun 09)'''
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*Check new small flasks of RBS
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'''TXG-->13Nov08-->XX'''
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**RBS (6A) - Try to spin down on Monday and Friday.
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**12Jun09 - Refresh 2 small flasks with the date of 17Apr09 and SD the other 4 flasks => next Monday (15Jun09) SD '''ALL''' RBS small flasks'''Done BD 26Jun09'''
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***Do not scrape. Just pour to avoid most of soil in spin down. Still do quick spin before starting normal spin down.
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*We will not spin down PLO or DIP until further notice.
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*Colorless Organism Refresh for 1Jul09:
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'''UEA-->13Nov08-->X'''
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**refresh PLC and ENT (on colorless organism sheet - get transferred every 3 weeks)'''  
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 +
'''SUB-->NODATE-->XXXX'''
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If not finished, the last person to leave should e-mail Katy
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'''MPI-->17APR09-->X'''
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'''TUH-->6MAR09-->X'''
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'''Priority:'''
 
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*ZG - update UGA flasks sheet as to what we have in flasks...there were a bunch of small flasks at the bottom of incubator 4 that were completely dried down and never transferred to big flasks, so SEJ tossed these and they shouldn't be on the list now unless there are big flasks for them
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=== List of Organisms needed by MSU ===
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*Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri'''Done BD 26Jun09'''
+
Please find these organisms and make an additional tube of them. If any are not alive or contaminated please indicate below next to the three letter codes, please email Katy with any questions
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*Wipe down all molecular work benches with the [[FIX]] 70% Ethanol squirt bottle every Wed & Fri'''Done BD 26Jun09'''
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'''Cultures:'''
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*JI & AI - work on eliminating backup backup racks in 2E
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*Check old flasks in 2E (5 large and 2 small). See if alive, still contaminated and with what they are contaminated. Post here on wiki. '''Done - CC; Only 3 alive; Checked tubes to see if alive or better than dead/contaminated flasks; Updated organism status in Culture Sheet; Made new flasks of contaminated EPE (tubes are dead); Need to SCI later 4/3/09'''
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*check flasks and '''spin down''' if ready... (don't forget the flasks in the #2 and #3 incubators)...switch over any of Sarah's in closed flasks into vented flasks
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'''Caitlin- here are the organisms you set out for me to transfer to small flask today (12 Jun 09)'''
+
-
'''TXG-->13Nov08-->XX'''
+
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'''UEA-->13Nov08-->X'''
+
-
'''SUB-->NODATE-->XXXX'''
+
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'''MPI-->17APR09-->X'''
+
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'''TUH-->6MAR09-->X'''
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 +
*CCM
 +
*EDS
 +
*EPE
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*ETM
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*ELU
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*HYO
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*KHQ
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*LXC
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*PET
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*SXD
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*SXE
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*SUL
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*TRT
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*TUB
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*TUV
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*TUY
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*TUX
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*TUM
 +
*TUP
 +
*TUO
 +
*LXB
 +
*LXF
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*LXH
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*LXJ
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*LXR
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*LXT
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*LYE
 +
*LYF
 +
*LYI
 +
*LUF
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*LUK
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*LUT
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*LUY
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*TSP
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'''Lab Assistants, Please Update Sarah/Christina On:'''
 
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*'''Updates for Sarah'''
 
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**list of all cultures I need in small flasks that have been started (any moved to big flasks yet?)
 
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**list of all cultures I need in small flasks that are contaminated (and preferably what they are contaminated with)
 
*'''Updates for Katy'''
*'''Updates for Katy'''

Revision as of 19:20, 3 September 2009

Contents

News

Wiki

  • Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
  • If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
  • You can upload pictures, word, excel, and pdf documents.
  • If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.


Cultures & Lab Announcements

Next lab assistants meeting TBA


Recap of lab assistants meeting Wednesday, September 2nd at 3:30 pm

  • Organisms to send to MSU
  • Organisms with contamination: PLO
  • Update inventory of chemicals for MSDS binder
  • Updates to the Wiki

Professional Enrichment

  • The Association of Women in Sciences (AWIS)


Things To Do

Priority

See the list of organisms needed by MSU below

Next Lab Meeting TBA

Week of Aug 31 - Sept 4

  • If you have any questions call/email Katy
  • Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri wed done TA/DO 2sept
  • Wipe down all molecular work benches with the FIX 70% Ethanol squirt bottle every Wed & Fri wed done TA/DO 2sept
  • The wiki is in the updating process if you're not sure what to do see Are you a Lab Assistant with free time on your hands?


Lets find out what happened to these cultures

Please email Katy if you find these cultures with the status and location Caitlin- here are the organisms you set out for me to transfer to small flask today (12 Jun 09)

TXG-->13Nov08-->XX

UEA-->13Nov08-->X

SUB-->NODATE-->XXXX

MPI-->17APR09-->X

TUH-->6MAR09-->X


List of Organisms needed by MSU

Please find these organisms and make an additional tube of them. If any are not alive or contaminated please indicate below next to the three letter codes, please email Katy with any questions

  • CCM
  • EDS
  • EPE
  • ETM
  • ELU
  • HYO
  • KHQ
  • LXC
  • PET
  • SXD
  • SXE
  • SUL
  • TRT
  • TUB
  • TUV
  • TUY
  • TUX
  • TUM
  • TUP
  • TUO
  • LXB
  • LXF
  • LXH
  • LXJ
  • LXR
  • LXT
  • LYE
  • LYF
  • LYI
  • LUF
  • LUK
  • LUT
  • LUY
  • TSP


  • Updates for Katy


Notes for Sarah

  • Euglenozoa AToL

Are you a Lab Assistant with free time on your hands?

  • keep up with washing, drying and autoclaving the glass volumetric pipets so we don't run out (5, 10, 25mL)
  • put cotton in the tops of pasteur pipets and fill the containers (by the dishwashing station) and autoclave them
  • Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
  • Autoclave media using the liquid cycle (be sure you label with autoclave tape)
  • Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
  • Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
  • Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
  • Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
  • Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
  • Make transfers of healthy cultures (all culture information can be found on the Cumulative Culture Inventory.xls sheet on the Farmer Lab PC desktop)
    • tubes
    • big/small flasks
  • Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
  • Extract DNA from concentrated cells and update the DNA.xls sheet
  • Qualify/Quantify DNA on Nanodrop
  • Maintain molecular buffers, reagents
  • Maintain at least one carboy of DI water
  • Keep everything clean and organized
  • Ask if anyone in the lab needs anything done



Lab Binder & Notebook

Phylogenetics and Systematics of Euglenozoa

Documents



See Also

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