Lab Knowledge Base

From Protists

Revision as of 19:59, 20 May 2009

News

Wiki

• Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
• If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
• You can upload pictures, word, excel, and pdf documents.
• If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.

Cultures & Lab Announcements

Professional Enrichment

• The Association of Women in Sciences (AWIS)

Pressing Things TO DO!

Next Lab Meeting (3Apr09 @ 2:30pm)

1. Updates on Sarah's culture stuff (i.e. Large flask status, backup tube status, incubator 2 stuff, backup backup racks)
2. Discuss new labels for test tubes.
3. Discuss Seawater.

Today (12May09)

Things that must be done today or tomorrow:

• call SEJ or CC if you have any questions (if not at lab, then working from home)

• Check large flasks of RBS and PLO and small flasks of DIP for spin downs.
  • PLO (6C) - Try to spin down on Monday and FridayDONE 18 MAY 09 BD
    • Scrape with sterile scraper. Do quick spin down first before starting normal spin down.
  • RBS (6A) - Try to spin down on Monday and Friday.
    • Do not scrape. Just pour to avoid most of soil in spin down. Still do quick spin before starting normal spin down.
    • Make new large flasks once a month.
    • the number of cells in large flasks are low, need to check Friday (15May09) Still no cells BD 20 may 09 Did not spin down
  • DIP (6D) - Spin down on Monday, Wednesday, and FridayDONE 20 MAY 09 BD
    • Start new flask before spin down (Make sure you know how to properly do it before you start). Do not scrape. Rock gently.
• Check that all new test tubes are doing well before throwing flasks out. done, need to make new test tubes from the big flasks based on how the new test tubes are doing
  • Spin down the flasks and toss them.
• Start checking test tube racks and update Sarah's Rack
• Colorless Organism Refresh for 20May09:
  • start new flasks for all colorless organisms that use media ATCC 802
  • refresh PLC and ENT too (on colorless organism sheet - get transferred every 3 weeks

If not finished, the last person to leave should e-mail SEJ or CC what's left to be done (depending on who it's for).

Priority:

• ZG - update UGA flasks sheet as to what we have in flasks...there were a bunch of small flasks at the bottom of incubator 4 that were completely dried down and never transferred to big flasks, so SEJ tossed these and they shouldn't be on the list now unless there are big flasks for them
• Empty all tip/trash box(s)/containers on gel and molecular benches every Wed & Fri
• Wipe down all molecular work benches with the FIX 70% Ethanol squirt bottle every Wed & Fri

Cultures:

• JI & AI - work on eliminating backup backup racks in 2E
• Check old flasks in 2E (5 large and 2 small). See if alive, still contaminated and with what they are contaminated. Post here on wiki. Done - CC; Only 3 alive; Checked tubes to see if alive or better than dead/contaminated flasks; Updated organism status in Culture Sheet; Made new flasks of contaminated EPE (tubes are dead); Need to SCI later 4/3/09
• check flasks and spin down if ready... (don't forget the flasks in the #2 and #3 incubators)...switch over any of Sarah's in closed flasks into vented flasks

Lab Assistants, Please Update Sarah/Christina On:

• Updates for Sarah
  • list of all cultures I need in small flasks that have been started (any moved to big flasks yet?)
  • list of all cultures I need in small flasks that are contaminated (and preferably what they are contaminated with)

• Updates for Christina

Notes for Sarah

• Euglenozoa AToL

Are you a Lab Assistant with free time on your hands?

• keep up with washing, drying and autoclaving the glass volumetric pipets so we don't run out (5, 10, 25mL)
• put cotton in the tops of pasteur pipets and fill the containers (by the dishwashing station) and autoclave them
• Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
• Autoclave media using the liquid cycle (be sure you label with autoclave tape)
• Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
• Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
• Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
• Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
• Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
• Make transfers of healthy cultures (all culture information can be found on the Cumulative Culture Inventory.xls sheet on the Farmer Lab PC desktop)
  • tubes
  • big/small flasks
• Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
• Extract DNA from concentrated cells and update the DNA.xls sheet
• Qualify/Quantify DNA on Nanodrop
• Maintain molecular buffers, reagents
• Maintain at least one carboy of DI water
• Keep everything clean and organized
• Ask if anyone in the lab needs anything done

Lab Binder & Notebook

• Protocols
• Media and Molecular Recipes
• Culture Inventory
• Inventory
• Instructional Information
• Recipes not Currently Used
• Culture Ordering Information

Phylogenetics and Systematics of Euglenozoa

• Euglenozoa Phylogenies
• Euglenophyte Phylogenies
• Phylogenetic Programs
• Farmer Lab

Documents

• PCR Reactions
• DNA Dilution Quick Calculation

See Also

• http://meta.wikimedia.org/wiki/Help:Wikitext_examples