Molecular Reagent Dilution Cheat Sheet

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! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
! Final Volume of Mix (uL)
! Final Volume of Mix (uL)
-
! Final Mix Concentration (total dNTPs)
+
! Working Stock Concentration (total dNTPs)
-
! Final Mix Concentration (each dNTP)
+
! Working Stock Concentration (each dNTP)
|-  
|-  
| 20
| 20
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|-
|-
|}
|}
 +
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! DMSO (uL)
! DMSO (uL)
! Final Volume of Mix (uL)
! Final Volume of Mix (uL)
-
! Final Mix Concentration
+
! Working Stock Concentration
|-  
|-  
| 5
| 5
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| 100
| 100
| 500x
| 500x
 +
|-
 +
|}
 +
 +
 +
 +
 +
*[[Oligo Primers]] - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
! Original Primer Mix (uL)
 +
! Autoclaved & Frozen HPLC H<sub>2</sub>O (uL)
 +
! Final Volume of Mix (uL)
 +
! Working Stock Concentration
 +
|-
 +
| 2
 +
| 98
 +
| 100
 +
| 10uM
 +
|-
 +
| 4
 +
| 196
 +
| 200
 +
| 10uM
 +
|-
 +
| 6
 +
| 294
 +
| 300
 +
| 10uM
 +
|-
 +
|}
 +
 +
 +
 +
 +
*[[Tricolor Loading Dye]] - this works for the current original stock we have, which is 5x and found in the -20C (be sure to use the same buffer to dilute this as you will be using to create your gel)
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
! Original 5x Loading Dye (uL)
 +
! 1x [[TBE]] or [[TAE]] buffer (uL)
 +
! Final Volume of Mix (uL)
 +
! Working Stock Concentration
 +
|-
 +
| 100
 +
| 400
 +
| 500
 +
| 1x
 +
|-
 +
| 200
 +
| 800
 +
| 1000
 +
| 1x
|-
|-
|}
|}

Current revision as of 02:05, 14 February 2008

  • dNTPs - this works for the current original stock we have, which is 100mM total dNTP concentration (25mM/dNTP) and found in the -20C
Original dNTP Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix (uL) Working Stock Concentration (total dNTPs) Working Stock Concentration (each dNTP)
20 30 50 40mM 10mM
40 60 100 40mM 10mM
60 90 150 40mM 10mM
80 120 200 40mM 10mM



  • Gelstar - this works for the current original stock we have 10,000x found in the -20C (in its own circular white container)
Original 10,000x Gelstar (uL) DMSO (uL) Final Volume of Mix (uL) Working Stock Concentration
5 95 100 500x



  • Oligo Primers - this works for the current original stock we have (water already added), which is 500uM and found in the -20C in the "original stock" box
Original Primer Mix (uL) Autoclaved & Frozen HPLC H2O (uL) Final Volume of Mix (uL) Working Stock Concentration
2 98 100 10uM
4 196 200 10uM
6 294 300 10uM



  • Tricolor Loading Dye - this works for the current original stock we have, which is 5x and found in the -20C (be sure to use the same buffer to dilute this as you will be using to create your gel)
Original 5x Loading Dye (uL) 1x TBE or TAE buffer (uL) Final Volume of Mix (uL) Working Stock Concentration
100 400 500 1x
200 800 1000 1x
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