1x Tris Buffer
From Protists
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**Graduated cylinder located under sink attached to the Nanodrop/Gel counter | **Graduated cylinder located under sink attached to the Nanodrop/Gel counter | ||
*Transfer the solution into a [[FIX]] 1L media bottle and label appropriately (10mM Tris, pH 8.0, your initials and the date). | *Transfer the solution into a [[FIX]] 1L media bottle and label appropriately (10mM Tris, pH 8.0, your initials and the date). | ||
| + | **[[FIX]] media bottles in cabinets above or below [[FIX]] heat/stir plate | ||
*[[Autoclave]] for 30min (since only 500mL). | *[[Autoclave]] for 30min (since only 500mL). | ||
*Let cool. | *Let cool. | ||
Revision as of 16:47, 3 December 2007
- In FIX graduated cylinder, add 50mL of the sterilized 10x Tris Buffer into 450mL dH2O.
- Graduated cylinder located under sink attached to the Nanodrop/Gel counter
- Transfer the solution into a FIX 1L media bottle and label appropriately (10mM Tris, pH 8.0, your initials and the date).
- Autoclave for 30min (since only 500mL).
- Let cool.
- Store at room temperature on the shelves above the molecular work bench.
- This makes 500mL of 1x TBE total (10mM Tris pH 8.0).
When this stock solution of 1x Tris Buffer runs low or out, make more 1x Tris Buffer from the 10x Tris Buffer and remake the 10x Tris Buffer first if that stock solution is out.
