10x Tris Buffer

From Protists

• Measure and pour ~300mL dH2O from the FIX graduated cylinder into a FIX 1L media bottle.
  • Graduated cylinder under sink attached to the Nanodrop/Gel counter
  • Media bottle in cabinets above or below FIX heating/stir plate
• Add 7.88g Tris-HCl to media bottle.
• Adjust pH to 8.0 with NaOH pellets.
• Pour mixture back into the FIX graduated cylinder and add dH2O up to 500mL.
• Pour mixture back into the FIX 1L media bottle and mix well.
• Label media bottle appropriately [10x (100mM) Tris, pH 8.0, your initials and the date].
• Autoclave for 30min (since only 500mL).
• Let cool.
• This makes 500mL of 10x TBE total (100mM Tris pH 8.0).
• Continue by following directions for 1x Tris Buffer, but store remaining 10x Tris Buffer in 1L media bottle contents at room temperature on shelves above molecular work bench.