10x TE
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| - | *add 1.219g Tris | + | *add 1.219g Tris to a [[FIX]] 100mL graduated cylinder |
*add 0.37g EDTA | *add 0.37g EDTA | ||
| - | *add up to 100mL dH2O | + | *add up to 100mL dH2O and mix well (cover with parafilm to prevent leakage) |
*check and adjust pH to 8.0 if necessary | *check and adjust pH to 8.0 if necessary | ||
| + | *makes a total of 100mL 10x TE | ||
| + | *store in small [[FIX]] media bottle under sink attached to the Nanodrop/Gel counter | ||
''Dilute 10x TE to working stock [[1x TE]] prior to its use in molecular work.'' | ''Dilute 10x TE to working stock [[1x TE]] prior to its use in molecular work.'' | ||
Current revision as of 15:43, 1 October 2007
- add 1.219g Tris to a FIX 100mL graduated cylinder
- add 0.37g EDTA
- add up to 100mL dH2O and mix well (cover with parafilm to prevent leakage)
- check and adjust pH to 8.0 if necessary
- makes a total of 100mL 10x TE
- store in small FIX media bottle under sink attached to the Nanodrop/Gel counter
Dilute 10x TE to working stock 1x TE prior to its use in molecular work.
