Lab Knowledge Base

From Protists

Revision as of 17:42, 19 September 2007

News

• Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
• If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
• You can upload pictures, word, excel, and pdf documents.
• If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
• The Association of Women in Sciences (AWIS)
  • About the Organization: AWIS brings together undergraduate and graduate women in the sciences to discuss science, careers and lifestyles in science as well as providing organized opportunities for science outreach in the local community. There are no dues and both men and women are encouraged to join! Contact Sana Hashmi (AWIS Undergraduate Presidentt) to be added to the AWIS listserve.
  • Outreach:
    • Dates:
      • September 27th, 3:30-4:30pm
      • October 18th, 3:30-4:30pm
      • November 15th, 3:30-4:30pm
    • Where: Oglethrope Elementary School (carpools are available)
    • What: helping elementary students conduct small science experiments
  • First Meeting of the Semester:
    • When: 6:30pm, September 17th
    • Where: SLC Room 269
    • Who: anyone who is interested!
    • What: AWIS informational meeting and discussion about being a science student at UGA. This will be a great opportunity for everyone to meet fellow science majors and discuss classes, good professors, and various other opportunities on campus.
  • First Seminar of the Semester:
    • When: time TBA, October 2nd
    • Where: TBA
    • Who: Dr. Maxia Dong, Center for Disease Control (CDC) and the National Institute of Health (NIH)
    • What: TBA

Pressing Things TO DO!

• ANNOUNCEMENT: Some of my lovely babies are growing!!! (Hooray!!) The Rhynchobodo sp. (RBS) are growing in a small flask in the 25C incubator. Please feel free to take a look at these new children of the lab. Also, please take a moment to mourn the loss of the other 2 ATCC cultures that came in (Diplonema ambulator and Trypanoplasma borreli). They did not survive past birth.

• LEARN HOW TO DO DNA Extraction Protocol...do it by yourself within ~1week (with Sarah or Tina backing you up)
  • make sure you take notes about the samples (where from in cell box, spin down date & person, which dna box #, etc.), anything that happened during the protocol, anything about the curves in when looking at the resulting DNA on the nanodrop, etc.

Cultures:

• tell Sarah status of these cultures in big flasks (or in general if they aren't in big flasks yet...start them in big flasks then. Make sure that the transfer to a big flask is from a small flask. If a small flask doesn't exist, please start one and use that for the big flasks)
  • S113 (SUA)
  • Strombomonas acuminata (SMA=SAC)
  • Trachelomonas scabra (T235? - TSB)
  • STB
  • SXA
  • Colacium mucronatum (MUR=MUC)
  • ELA
  • LSG
  • TET
• on Monday the 17th - check the new Eutreptiella cultures...tubes & flasks (15C incubator, 20C incubator in Eutreptia pertyi rack, and in rack in front of window by the Farmer Lab PC)...report to Sarah results and which media/temps the organisms like best (has anyone done this?...please email me the results)
• start growing the MSU cultures up in a small flasks in addition to the back up tube (recently added to the culture inventory...some might already be in their appropriate rack number if they are just replacing a culture we had previously)...will need lots of AF6
• on Monday the 17th check the GRC small flasks...2 total (we need to extract more DNA from this, so don't just toss it w/out spinning down if in log-phase growth & enough cells)...Sarah checked today, still in log, but 2nd flask from 13Sept07 not grown up enough...wait a little longer
• check big flasks & spin down if necessary (all done?)
• check back up small flasks & "no cells" small flasks in the culture room 20C incubator (transfers made or back ups checked & ok?)
• check on latest transfers of ENTO, PER (done...it's dead...re-ordering), Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
• make up more K & Seawater Media - MSU (the AF6 with seawater)...use multiple containers for each type of media b/c some will have to be kept at room temp. and others will have to be kept in the 15C incubator (done yet?)
• Brandon will be doing fungal treatment experiments using Sharan's notes in Lab Binder

Other:

• update chemical inventory with the chemicals whose MSDS sheets are next to the Farmer Lab PC and then put the MSDS sheets away alphabetically in the MSDS binder
• drill two extra holes in the black drying rack for the culture room and mount it on the wall above the current dishwashing area (move all glassware and dishwashing items first so they don't get broken or in the way)...at least 2-3 people will be needed to lift and mount the drying rack
• move stuff from molecular/PCR area and clean off top shelves...wipe of lower bench with ethanol when done and then replace all the stuff that was moved off temporarily (check with Tina and Sarah first to be sure they don't need to work on the bench while you are doing it)
• update culture inventory with which racks recently added MSU cultures are actually in (edit if some are repeats)
• wash dishes (make sure dry before putting away!)
• set up new answering machine to lab phone (with personalized message!)
• clean centrifuge rotor (read manuals for how to do this...the one next to it from the Keith lab)
• hang up SEJ's poster (& Sheila's when she brings it in)

Are you a Lab Assistant with free time on your hands?

• Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
• Autoclave media using the liquid cycle (be sure you label with autoclave tape)
• Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
• Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
• Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
• Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
• Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
• Make transfers of healthy cultures
  • tubes
  • big/small flasks
• Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
• Extract DNA from concentrated cells
• Qualify/Quantify DNA on Nanodrop
• Maintain molecular buffers, reagents
• Maintain at least one carboy of DI water
• Keep everything clean and organized
• Ask if anyone in the lab needs anything done

Lab Binder & Notebook

• Protocols
• Media Recipes
• Culture Inventory
• Inventory
• Instructional Information
• Recipes not Currently Used
• Culture Ordering Information

Phylogenetics and Systematics of Euglenozoa

• Euglenozoa Phylogenies
• Euglenophyte Phylogenies
• Phylogenetic Programs

Documents

• PCR Reactions
• DNA Dilution Quick Calculation

See Also

• http://meta.wikimedia.org/wiki/Help:Wikitext_examples