Lab Knowledge Base
From Protists
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'''Cultures:''' | '''Cultures:''' | ||
| + | *start growing the MSU cultures up in a small flasks in addition to the back up tube (recently added to the culture inventory...some might already be in their appropriate rack number if they are just replacing a culture we had previously)...will need lots of AF6 | ||
*GRC ready to be transferred/spun down? (we need to extract more DNA from this, so don't just toss it w/out spinning down if in log-phase growth & enough cells) | *GRC ready to be transferred/spun down? (we need to extract more DNA from this, so don't just toss it w/out spinning down if in log-phase growth & enough cells) | ||
*check big flasks & spin down if necessary '''(all done?)''' | *check big flasks & spin down if necessary '''(all done?)''' | ||
*check back up small flasks & "no cells" small flasks in the culture room 20C incubator '''(transfers made or back ups checked & ok?)''' | *check back up small flasks & "no cells" small flasks in the culture room 20C incubator '''(transfers made or back ups checked & ok?)''' | ||
*check on latest transfers of ENTO, PER '''(done...it's dead...re-ordering)''', ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!) | *check on latest transfers of ENTO, PER '''(done...it's dead...re-ordering)''', ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!) | ||
| - | *how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary ''' | + | *how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary '''ok for now, but will have to make up some more if the cultures start growing rapidly''' |
'''Other:''' | '''Other:''' | ||
| - | *update culture inventory with MSU cultures | + | *refill pipet tip boxes & microfuge tube containers with appropriate tubes & autoclave them |
| + | *refill empty agarose tubes with ~amount that label says (0.4mg, 0.6mg, 0.7mg) | ||
| + | *put items on cart in hall away in appropriate places | ||
| + | *update culture inventory with which racks recently added MSU cultures are actually in (edit if some are repeats) | ||
*wash dishes (make sure dry before putting away!) | *wash dishes (make sure dry before putting away!) | ||
*set up new answering machine to lab phone '''(with personalized message!)''' | *set up new answering machine to lab phone '''(with personalized message!)''' | ||
| - | *clean centrifuge rotors (read manuals for how to do this... | + | *clean centrifuge rotors (read manuals for how to do this...the one next to it from the Keith lab) |
*hang up SEJ's poster (& Sheila's when she brings it in) | *hang up SEJ's poster (& Sheila's when she brings it in) | ||
Revision as of 19:20, 11 September 2007
Contents |
News
- Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
- If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
- You can upload pictures, word, excel, and pdf documents.
- If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
- Informational Seminar in Research:
- When: 12pm (noon), September 12th
- Where: Room H236 at the College of Veterinary Medicine
- Who: all students are welcome to hear Dr. Khanna, DVM, PhD, Dip. ACVIM (oncology) and Dr. Melissa Paoloni, DVM, Dip. ACVIM (oncology)
- What: The Drs. will speak with students about a career in research, what is NIH, what is NCI, what it is like to work at NIH, opportunities at NIH, and how to get started in the research field.
- The Association of Women in Sciences
- About the Organization: AWIS brings together undergraduate and graduate women in the sciences to discuss science, careers and lifestyles in science as well as providing organized opportunities for science outreach in the local community. There are no dues and both men and women are encouraged to join! Contact Sana Hashmi (AWIS Undergraduate Presidentt) to be added to the AWIS listserve.
- Outreach:
- Dates:
- September 27th, 3:30-4:30pm
- October 18th, 3:30-4:30pm
- November 15th, 3:30-4:30pm
- Where: Oglethrope Elementary School (carpools are available)
- What: helping elementary students conduct small science experiments
- Dates:
- First Meeting of the Semester:
- When: 6:30pm, September 17th
- Where: SLC Room 269
- Who: anyone who is interested!
- What: AWIS informational meeting and discussion about being a science student at UGA. This will be a great opportunity for everyone to meet fellow science majors and discuss classes, good professors, and various other opportunities on campus.
- First Seminar of the Semester:
- When: time TBA, October 2nd
- Where: TBA
- Who: Dr. Maxia Dong, Center for Disease Control (CDC) and the National Institute of Health (NIH)
- What: TBA
Pressing Things TO DO!
- LEARN HOW TO DO DNA Extraction Protocol...do it by yourself within ~1week (with Sarah or Tina backing you up)
- transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)
Cultures:
- start growing the MSU cultures up in a small flasks in addition to the back up tube (recently added to the culture inventory...some might already be in their appropriate rack number if they are just replacing a culture we had previously)...will need lots of AF6
- GRC ready to be transferred/spun down? (we need to extract more DNA from this, so don't just toss it w/out spinning down if in log-phase growth & enough cells)
- check big flasks & spin down if necessary (all done?)
- check back up small flasks & "no cells" small flasks in the culture room 20C incubator (transfers made or back ups checked & ok?)
- check on latest transfers of ENTO, PER (done...it's dead...re-ordering), Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
- how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary ok for now, but will have to make up some more if the cultures start growing rapidly
Other:
- refill pipet tip boxes & microfuge tube containers with appropriate tubes & autoclave them
- refill empty agarose tubes with ~amount that label says (0.4mg, 0.6mg, 0.7mg)
- put items on cart in hall away in appropriate places
- update culture inventory with which racks recently added MSU cultures are actually in (edit if some are repeats)
- wash dishes (make sure dry before putting away!)
- set up new answering machine to lab phone (with personalized message!)
- clean centrifuge rotors (read manuals for how to do this...the one next to it from the Keith lab)
- hang up SEJ's poster (& Sheila's when she brings it in)
Are you a Lab Assistant with free time on your hands?
- Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
- Autoclave media using the liquid cycle (be sure you label with autoclave tape)
- Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
- Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
- Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
- Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
- Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
- Make transfers of healthy cultures
- tubes
- big/small flasks
- Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
- Extract DNA from concentrated cells
- Qualify/Quantify DNA on Nanodrop
- Maintain molecular buffers, reagents
- Maintain at least one carboy of DI water
- Keep everything clean and organized
- Ask if anyone in the lab needs anything done
Lab Binder & Notebook
- Protocols
- Media Recipes
- Inventory
- Instructional Information
- Recipes not Currently Used
- Culture Ordering Information
Phylogenetics and Systematics of Euglenozoa
Documents
