Lab Knowledge Base

(Difference between revisions)

Revision as of 13:15, 31 August 2007

Welcome to the Lab Wiki for Mark Farmer's Lab. This is the place to find procedures, news, and answers to commonly asked questions.
If you are new to this wiki, you will need an account in order to edit pages. Talk to Sarah about getting an account set up if you would like to contribute.
You can upload pictures, word, excel, and pdf documents.
If you are new to wiki editing, check out the wiki help for how to edit articles in a wiki, then use the existing pages as templates and examples of how to create links, upload files, and display images.
WELCOME BACK STUDENTS AND GOOD LUCK THIS FALL SEMESTER!!!

tell SEJ about any supplies that we are out of/running low on so she can purchase them
transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)

Friday - Caitlin and Joy

keep checking test tube racks (can work ahead) and then transfer them (Stephen made 3L of AF6, so ready to go)
check big flasks & spin down if necessary
check back up small flasks & "no cells" small flasks in the culture room 20C incubator
check on ENTO, PER, Eutreptia pertyi, and Eutreptiella sp. cultures (also TET in walk in, but be sure not to keep out long!)
make transfers of the best ENTO, PER, TET and if Eutreptia pertyi and Eutreptiella sp. are ready, transfer them too
how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary

Other:

update culture inventory with MSU cultures and the 3letter codes SEJ gave them
wash dishes
move empty boxes under black table in culture room and break down
move everything off of black table (carboys can go under big black table in the transfer hood room)
move small black table into the transfer hood room (end to end with the other big black table)...will need at least two people b/c have to take off black top (w/ power drill), move base and top separately into transfer hood room and then reassemble the table
hook up and set up new answering machine to lab phone
label tops of balance tubes with uL in each (for easy spinning balance reference)
clean centrifuge rotors (read manuals for how to do this...5417C and the one next to it from the Keith lab)
hang up SEJ's poster (& Sheila's when she brings it in)

Make any media that needs to be made (generally AF-6 Medium, ESNW or Hay Infusion)
Autoclave media using the liquid cycle (be sure you label with autoclave tape)
Using sterile technique, add any additional ingredients to complete media post-autoclaving (eg. vitamins)
Wash test tubes, media containers and their respective caps (keep LIVE and FIX glassware separated)
Autoclave the test tubes, media containers, 9" pasteur pipets, pipet tip box(es), volumetric pipets, etc. in appropriate containers with containers underneath if available
Using sterile techniqe, add media to the autoclaved tubes or sterile culture flasks
Check and make notes of cultures without opening the tubes or culture flasks using the appropriate microscopes. Some cultures need to be checked daily, weekly, bi-weekly or monthly depending on their growth patterns.
Make transfers of healthy cultures
- tubes
- big/small flasks
Spin down log phase cells in big culture flasks and rejuvenate with fresh media (aseptically)
Extract DNA from concentrated cells
Qualify/Quantify DNA on Nanodrop
Maintain molecular buffers, reagents
Maintain at least one carboy of DI water
Keep everything clean and organized
Ask if anyone in the lab needs anything done

@@ Line 10: / Line 10: @@
 *tell SEJ about any supplies that we are out of/running low on so she can purchase them
 *transfer all back up test tube racks! (already started so check in notebook to see where the last person left off)
-*check on ENTO, PER, ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!)
 '''Friday - Caitlin and Joy'''
-*keep checking test tube racks (can work ahead) and then transfer them when new AF6 cooled enough
+*keep checking test tube racks (can work ahead) and then transfer them (Stephen made 3L of AF6, so ready to go)
 *check big flasks & spin down if necessary
 *check back up small flasks & "no cells" small flasks in the culture room 20C incubator
+*check on ENTO, PER, ''Eutreptia pertyi'', and ''Eutreptiella'' sp. cultures (also TET in walk in, but be sure not to keep out long!)
+*make transfers of the best ENTO, PER, TET and if ''Eutreptia pertyi'' and ''Eutreptiella'' sp. are ready, transfer them too
+*how much K & Seawater Media - MSU (the AF6 with seawater do we have? (we are going to be getting some replacement Eutreptiella cultures that will need these mediums soon)...make them up if necessary