50x TAE

From Protists

• Measure and pour ~700mL dH2O from the FIX graduated cylinder into a FIX large flask
  • Graduated cylinder under sink attached to the Nanodrop/Gel counter
  • Large flask in cabinets above FIX heating/stir plate
• Add 18.61g Na2EDTA x 2H20 (MW 372.24)
  • In chemical shelves alphabetically as "EDTA disodium salt, dihydrate, crystal"
• Add 57.1mL Glacial Acetic Acid (CH3COOH) in hood with blower on (Use Gloves and Goggles!)
  • Glacial Acetic Acid is in the Acid Storage container under the hood across from the Nanodrop/Gel Image counter
• Add 242.0g Tris Base (or TRIZMA base)
• Put a FIX stir bar in the large flask and cover lid with aluminum foil so dirt/dust doesn't get in mixture
• Put flask on the stir plate and allow to stir until all Tris Base and Na2EDTA is dissolved
• Pour mixture back into the FIX graduated cylinder and add dH2O up to 1000mL
• Pour mixture back into the FIX large flask and mix well
• Makes a total of 1L of 50x TAE buffer
• Store in 1L media bottles with orange caps under sink attached to the Nanodrop/Gel counter

Dilute the 50x TAE buffer to 1X TAE before using in gels or other molecular work