Designing Primers

From Protists

Background of Primers

Primers are used in pairs to help you isolate a specific gene or region of a gene of interest from an organism's genomic DNA.

Designing Primers

• Designing Non-Degenerate Primers
• Designing Degenerate Primers

Helpful Links

• [Wikipedia PCR article http://en.wikipedia.org/wiki/Polymerase_chain_reaction]
• [Narrated animation of PCR http://www.sumanasinc.com/webcontent/animations/content/pcr.html] from the educational multimedia company Sumanas.
• [Animation of PCR http://www.dnalc.org/ddnalc/resources/pcr.html] from Cold Spring Harbor's Dolan DNA Learning Center.