Snow Algae

From Protists

Background

These cultures were originally from Ron Hoham and are currently in the UTEX culture collection. We were storing them and maintaining them for use in a collaboration between Ron Hoham (now retired from Colgate) and Dr. Jim Leebens-Mack (plant systematist at UGA). However, most were contaminated with fungi and/or lost because they were killed when the walk-in broke over the summer of 2007 and/or they were not transferred often enough and the agar dried out.

Snow Algae Media Recipe

Snow Algae Agar Plates

• measure out 525mL Snow Algae Media (above) into a LIVE graduated cylinder
• add 10.5g of Agar (not agarose!!) to make a 2% weight/volume agar concentration
• distribute the mixture into a LIVE media bottle(s)
• heat the mixture on the LIVE hot/stir plate to dissolve the agar completely
• Autoclave the mixture in the bottles on the liquid cycle for the appropriate time