Autoclave

From Protists

REMEMBER: When you autoclave, make sure that you leave all caps or coverings slightly unscrewed or loosened so that pressure won't build up inside the tubes or bottles. When finished autoclaving, immediately tighten all caps/covers to prevent contamination. However, DO NOT overtighten or we will not be able to loosen the cap later when it is cooled (if this happens, leave stuck-shut glassware in the transfer hood while working with the flame on for awhile and it should warm up a little to make it easier to loosen). If you forget to loosen your caps, your glassware will explode and break the autoclave. The same goes for anything with aluminum foil...don't tighten it until it has finished autoclaving.