Notes for Sarah

From Protists

• Clarifications (EVI in racks 17 & 19, EVI & ESL possibly mixed up in rack 17...transfers or just tubes misplaced?)...do SSU if possible on both to dbl check
• couldn't finish this transfer b/c couldn't find any ETV [autoclave some distilled water when autoclaving other media and add this 1:1 with the newly made saltwater media (in sterile hood) and transfer some Eutreptia viridis (ETV) into a test tube and a small flask with this brackish (1/2 concentration of full strength seawater) mix of the media...(Joy worked on media)]
• ELU dies in K media, but also wasn't transferred as much...tossed and just use saltwater AF6 from now on
• ELU prefers to be at room temperature (RT) in the saltwater media (MSU), but K media works too (back up tubes & flasks in 20C)
• ETM (15C) and ELG (20C) also seem to prefer saltwater media (MSU), but K media works too
• T411=THC tubes appear to be only little round green things (LRGTs), i.e. green algae & not Euglenids (please inform SEJ if you see any Trachelomonas cells in any of these tubes so they can be saved and single cell isolated!)
• 31Oct07 realized some confusion over Euglena/Lepocinclis tripteris (TRP)...check BMa's original culture information to see if it was the same number and it has since been reclassified as a Lepocinclis rather than a Euglena...plus, it is probably dead @ UGA at this point if we didn't get it shipped from MSU...don't know where it is...
• apparently we don't have any ETV (Eutreptia viridis anymore...someone just mislabeled it as such but it was really EVI, Euglena viridis epitype)
• corning plastic flasks are the least toxic to our organisms (from culture collection experiments)
• the big flask of LAM is contaminated with another euglenid organism (see "Lab assistants please update SEJ/CC on" section)
  • check original tube cultures of LAM to see if they are contaminated with the round, big fat euglenid too...new small flask from test tube CS 25 Jan 08
  • if no, then:
    • start new small flask from uncontaminated tube culture of LAM
    • throw out old (contaminated) big flask of LAM
  • if yes, then:
    • single cell isolate LAM into a 6-well plate and start new tube and small flask
    • keep both contaminated tubes and contaminated big flasks of LAM as back-ups in case the single cell isolations don't work

New DNA as of mid-Feb:

• • TVL (cell box 1 - 05Dec06, EV, 0.0443 g, L, so ok)Done BP 02/15/08
  • ETM (cell box 2 - 10Oct07, CS, 0.157 g, L, so ok)Done 02/15/08
  • SNB (cell box 4 - check info on tubes prior to combining)(Done, ST 16Feb07)
  • TUX (cell box 7 - check info on tubes prior to combining, but if ok, can combine all 4 tubes in one extraction tube)(Done, ST 16Feb07)
  • TUK (cell box 7 - check info on tubes prior to combining, but if ok, can combine all 4 tubes in one extraction tube)(Done, ST 16Feb07)
  • TUV (cell box 9 - check info on tubes prior to combining)(Done, ST 16Feb07)
  • TSP (cell box 9 - check info on tubes prior to combining)(Done, ST 16Feb07)

• can't find small flask of EPE, the big flask of EPE looks gross, should I start a new small flask? YES!!- finished, but not a lot of cells in test tubes (CS).