From Protists

Soil Extract

Make the Soil Extract as follows:

1. Put 105 g of soil and 660 mL of deionized water into a 1L-bottle.
2. Autoclave for 15 minutes at 15 psi on 2 consecutive days.
3. Let contents settle for ~1 week.
4. Decant and filter the supernatant liquid.
5. Adjust pH to 7.0-8.0 with 1M HCl or 1M NaOH, if necessary.
6. Store in Media Cabinet as “CCAP Soil Extract Soln”. (Same as Soil Extract from CCAP ASWP)

Stock Solutions

There are three separate stock solutions made in distilled H2O in this media.

(1) K2HPO4	1.0g/L
(2) MgSO4.7H2O	1.0g/L
(3) KNO3	10.0g/L

Label appropriately with concentration and date on the bottles.
Store in 4C walk-in.

Soil Extract w/ Added Salts

Make this in a large flask as follows:

Stock Soln (1)	20.0 mL
Stock Soln (2)	20.0 mL
Stock Soln (3)	20.0 mL	CCAP Soil Extract Soln	100.0 mL	Distilled H2O	840.0 mL

Cover with foil.
Autoclave for 15 minutes at 15 psi.