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	= Final Concentration of 100 mg/mL Chloramphenicol: =		= Final Concentration of 100 mg/mL Chloramphenicol: =
-	#Add 20 mL of nuclease-free H2O to a 50 mL centrifuge tube	+	#Add 20 mL of nuclease-free H2O (found in the -20 freezer) to a 50 mL centrifuge tube
	#Add 30 mg of Chloramphenicol to dH2O		#Add 30 mg of Chloramphenicol to dH2O
	#Vortex solution until mixed		#Vortex solution until mixed

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Revision as of 19:57, 27 February 2009

Final Concentration of 100 mg/mL Chloramphenicol:

1. Add 20 mL of nuclease-free H2O (found in the -20 freezer) to a 50 mL centrifuge tube
2. Add 30 mg of Chloramphenicol to dH2O
3. Vortex solution until mixed
4. Aliquot solution: 1 mL of the final concentration of 100mg/mL Chloramphenicol into 1.5 mL micro centrifuge tubes
5. Label tubes with a C on the side and top of the tube
6. Place aliquoted solution tubes Tina's box on the bottom shelf of the -20 freezer.

Order Information:

• Fisher Scientific
• 100 g
• Catalog #: BP904-100