CCAP Per
From Protists
(Difference between revisions)
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= Soil Extract = | = Soil Extract = | ||
Make the Soil Extract as follows: | Make the Soil Extract as follows: | ||
| Line 39: | Line 38: | ||
|Stock Soln (3) | |Stock Soln (3) | ||
|20.0 mL | |20.0 mL | ||
| + | |- | ||
|CCAP Soil Extract Soln | |CCAP Soil Extract Soln | ||
|100.0 mL | |100.0 mL | ||
| + | |- | ||
|Distilled H2O | |Distilled H2O | ||
|840.0 mL | |840.0 mL | ||
| + | |- | ||
|} | |} | ||
Cover with foil.<br> | Cover with foil.<br> | ||
Autoclave for 15 minutes at 15 psi. | Autoclave for 15 minutes at 15 psi. | ||
Revision as of 16:18, 26 September 2007
Soil Extract
Make the Soil Extract as follows:
- Put 105 g of soil and 660 mL of deionized water into a 1L-bottle.
- Autoclave for 15 minutes at 15 psi on 2 consecutive days.
- Let contents settle for ~1 week.
- Decant and filter the supernatant liquid.
- Adjust pH to 7.0-8.0 with 1M HCl or 1M NaOH, if necessary.
- Store in Media Cabinet as “CCAP Soil Extract Soln”. (Same as Soil Extract from CCAP ASWP)
Stock Solutions
There are three separate stock solutions made in distilled H2O in this media.
| (1) K2HPO4 | 1.0g/L |
| (2) MgSO4.7H2O | 1.0g/L |
| (3) KNO3 | 10.0g/L |
Label appropriately with concentration and date on the bottles.
Store in 4C walk-in.
Soil Extract w/ Added Salts
Make this in a large flask as follows:
| Stock Soln (1) | 20.0 mL |
| Stock Soln (2) | 20.0 mL |
| Stock Soln (3) | 20.0 mL |
| CCAP Soil Extract Soln | 100.0 mL |
| Distilled H2O | 840.0 mL |
Cover with foil.
Autoclave for 15 minutes at 15 psi.
