Washing Protocol for Live Glassware
From Protists
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| - | Notes | + | == Notes == |
| - | *Only wash dishes using [[LIVE]] sinks and cleaning supplies such as brushes!!! | + | |
| - | *Change soapy rinse in tupperware container #1 after each use, but only change | + | |
| + | *Only wash dishes using [[LIVE]] sinks and cleaning supplies such as brushes and soap!!! | ||
| + | *Change soapy rinse in tupperware container #1 after each use, but only change RO rinses in tupperware containers #2 and #3 if they have been used 3 or more times or if they begin to look dirty | ||
| - | + | == Protocol == | |
| - | + | ||
| - | + | ||
| - | + | #Rinse out glassware with tap water until most large particulates are removed | |
| - | + | #Put tap water in glassware tupperware container #1 or directly into large pieces of glassware | |
| - | + | #Put 1-2 scoopfuls of the special soap near the sink directly into large glassware or into the tupperware container #1 (more soap is located underneath the sink in the culture room) | |
| - | + | #Allow the glassware to soak for 15-30min if there is not much residue left from cultures or media, or 1hr-overnight for glassware with lots of residue | |
| - | + | #Scrub the glassware thoroughly until all residue is removed and be sure to remove any labels | |
| - | + | #Rinse the glassware 3x's with tap water to remove any remaining soap | |
| - | + | #Put [http://editthis.info/protists/DI_water#Types_of_Water RO] water in glassware tupperware container #2 or directly into large pieces of glassware for 15-30min | |
| - | + | #Remove RO water from glassware (down drain or back in tupperware container #2) | |
| - | + | #Rinse off glassware with fresh RO water if necessary | |
| - | + | #Put RO water in glassware tupperware container #3 or directly into large pieces of glassware for 15-30min | |
| + | #Invert glassware on diaper paper and allow to dry | ||
| + | #[[Autoclave]] on gravity/dry cycle if the glassware is dried completely or on liquid cycle (30min sterilization) if there is still moisture in glassware | ||
| + | #Store clean/autoclaved glassware in cabinet in the culture room if they are not required immediately (leave autoclave indicator tape on them until they are used) | ||
Current revision as of 16:04, 18 April 2010
Notes
- Only wash dishes using LIVE sinks and cleaning supplies such as brushes and soap!!!
- Change soapy rinse in tupperware container #1 after each use, but only change RO rinses in tupperware containers #2 and #3 if they have been used 3 or more times or if they begin to look dirty
Protocol
- Rinse out glassware with tap water until most large particulates are removed
- Put tap water in glassware tupperware container #1 or directly into large pieces of glassware
- Put 1-2 scoopfuls of the special soap near the sink directly into large glassware or into the tupperware container #1 (more soap is located underneath the sink in the culture room)
- Allow the glassware to soak for 15-30min if there is not much residue left from cultures or media, or 1hr-overnight for glassware with lots of residue
- Scrub the glassware thoroughly until all residue is removed and be sure to remove any labels
- Rinse the glassware 3x's with tap water to remove any remaining soap
- Put RO water in glassware tupperware container #2 or directly into large pieces of glassware for 15-30min
- Remove RO water from glassware (down drain or back in tupperware container #2)
- Rinse off glassware with fresh RO water if necessary
- Put RO water in glassware tupperware container #3 or directly into large pieces of glassware for 15-30min
- Invert glassware on diaper paper and allow to dry
- Autoclave on gravity/dry cycle if the glassware is dried completely or on liquid cycle (30min sterilization) if there is still moisture in glassware
- Store clean/autoclaved glassware in cabinet in the culture room if they are not required immediately (leave autoclave indicator tape on them until they are used)
