EDTA
From Protists
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#Let cool. | #Let cool. | ||
#Store at room temperature on the shelves above the molecular work station. | #Store at room temperature on the shelves above the molecular work station. | ||
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==Links== | ==Links== | ||
[http://en.wikipedia.org/wiki/EDTA EDTA on Wikipedia] | [http://en.wikipedia.org/wiki/EDTA EDTA on Wikipedia] |
Current revision as of 16:26, 3 December 2007
Background
- EDTA is a widely-used abbreviation for the chemical compound ethylenediaminetetraacetic acid (also known by other names).
- EDTA binds heavy metals (such as Ca2+ and Mg2+ ions).
- In biochemistry and molecular biology ion depletion is commonly used to inactivate metal-dependent enzymes which could damage DNA or proteins.
- Therefore, EDTA helps to maintain our DNA for long-term storage.
0.5M EDTA Recipe
- Add 93g of EDTA into 300mL of dH2O (measure in a graduated cylinder, then transfer into a 1L media bottle...don't wash the graduated cylinder yet!).
- Adjust pH to 8.0 with NaOH pellets at which point the EDTA will go into solution.
- Pour the contents of the 1L media bottle back into the graduated cylinder used in step 1 and adjust volume to 500mL with dH2O.
- Pour the 500mL of the 0.5M (500mM) EDTA back into the 1L media bottle and label (chemical name, concentration, your initials and the date).
- Autoclave for 30min (since it is 500mL).
- Let cool.
- Store at room temperature on the shelves above the molecular work station.