CCAP Per
From Protists
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= Soil Extract = | = Soil Extract = | ||
Make the Soil Extract as follows: | Make the Soil Extract as follows: | ||
| Line 11: | Line 10: | ||
= Stock Solutions = | = Stock Solutions = | ||
| - | There are three separate stock solutions made in distilled H2O in this | + | There are three separate stock solutions made in distilled H2O in this medium. |
| + | |||
| + | {| | ||
| + | |(1) K2HPO4 | ||
| + | |1.0g/L | ||
| + | |- | ||
| + | |(2) MgSO4.7H2O | ||
| + | |1.0g/L | ||
| + | |- | ||
| + | |(3) KNO3 | ||
| + | |10.0g/L | ||
| + | |} | ||
| + | |||
| + | Label appropriately with concentration and date on the bottles.<br> | ||
| + | Store in 4C walk-in. | ||
| + | |||
| + | = Soil Extract w/ Added Salts (SES) = | ||
| + | Make this in a large flask as follows: | ||
| + | |||
| + | {| | ||
| + | |Stock Soln (1) | ||
| + | |20.0 mL | ||
| + | |- | ||
| + | |Stock Soln (2) | ||
| + | |20.0 mL | ||
| + | |- | ||
| + | |Stock Soln (3) | ||
| + | |20.0 mL | ||
| + | |- | ||
| + | |CCAP Soil Extract Soln | ||
| + | |100.0 mL | ||
| + | |- | ||
| + | |Distilled H2O | ||
| + | |840.0 mL | ||
| + | |- | ||
| + | |} | ||
| - | |||
| - | |||
| - | |||
| - | + | *There should be 1L total. | |
| + | *Cover the flask opening with foil. | ||
| + | *Autoclave for 15 minutes at 15 psi. | ||
| - | = | + | = CCAP Per Medium = |
| - | + | *Once the SES cools slightly, add 1.0g of Complan (in Media cabinet). | |
| + | *Mix briefly by swirling. | ||
| + | *Distribute into media bottles labelled "CCAP Per". | ||
| + | *Autoclave for 15 minutes at 15 psi. | ||
| + | *Store in Media cabinet. | ||
Current revision as of 16:26, 26 September 2007
Contents |
Soil Extract
Make the Soil Extract as follows:
- Put 105 g of soil and 660 mL of deionized water into a 1L-bottle.
- Autoclave for 15 minutes at 15 psi on 2 consecutive days.
- Let contents settle for ~1 week.
- Decant and filter the supernatant liquid.
- Adjust pH to 7.0-8.0 with 1M HCl or 1M NaOH, if necessary.
- Store in Media Cabinet as “CCAP Soil Extract Soln”. (Same as Soil Extract from CCAP ASWP)
Stock Solutions
There are three separate stock solutions made in distilled H2O in this medium.
| (1) K2HPO4 | 1.0g/L |
| (2) MgSO4.7H2O | 1.0g/L |
| (3) KNO3 | 10.0g/L |
Label appropriately with concentration and date on the bottles.
Store in 4C walk-in.
Soil Extract w/ Added Salts (SES)
Make this in a large flask as follows:
| Stock Soln (1) | 20.0 mL |
| Stock Soln (2) | 20.0 mL |
| Stock Soln (3) | 20.0 mL |
| CCAP Soil Extract Soln | 100.0 mL |
| Distilled H2O | 840.0 mL |
- There should be 1L total.
- Cover the flask opening with foil.
- Autoclave for 15 minutes at 15 psi.
CCAP Per Medium
- Once the SES cools slightly, add 1.0g of Complan (in Media cabinet).
- Mix briefly by swirling.
- Distribute into media bottles labelled "CCAP Per".
- Autoclave for 15 minutes at 15 psi.
- Store in Media cabinet.
