ATCC 802
From Protists
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#Make the media solution as follows: | #Make the media solution as follows: | ||
#*Cerophyll / Cereal Leaves '''2.5 g''' | #*Cerophyll / Cereal Leaves '''2.5 g''' | ||
#*Distilled H2O '''1.0 L''' | #*Distilled H2O '''1.0 L''' | ||
| - | #**Add the cerophyll to the distilled water in a flask. | + | #**Add the cerophyll to the distilled water in a 2L-flask or beaker. |
#**Add a stir bar and cover the opening of the flask with foil. | #**Add a stir bar and cover the opening of the flask with foil. | ||
#**Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10). | #**Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10). | ||
| Line 13: | Line 11: | ||
#**Autoclave for 15 minutes at 121°C. | #**Autoclave for 15 minutes at 121°C. | ||
#**Label as “ATCC 802” and store in Media cabinet. | #**Label as “ATCC 802” and store in Media cabinet. | ||
| - | #24 hours prior to inoculation, add the appropriate bacteria (indicated on | + | #24 hours prior to inoculation, add the appropriate bacteria (indicated on “[[Colorless Organisms]]” list) for the organism to the media in the tissue flask for culturing. |
#Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist. | #Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist. | ||
Current revision as of 18:48, 21 February 2008
- Make the media solution as follows:
- Cerophyll / Cereal Leaves 2.5 g
- Distilled H2O 1.0 L
- Add the cerophyll to the distilled water in a 2L-flask or beaker.
- Add a stir bar and cover the opening of the flask with foil.
- Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10).
- Add 20 mL of distilled H2O to compensate for evaporation.
- Filter through Whatman #1 filter paper.
- Add 0.94 g of Na2HPO4.7H2O to the solution.
- Distribute the media into media bottles for autoclaving.
- Autoclave for 15 minutes at 121°C.
- Label as “ATCC 802” and store in Media cabinet.
- 24 hours prior to inoculation, add the appropriate bacteria (indicated on “Colorless Organisms” list) for the organism to the media in the tissue flask for culturing.
- Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
