ATCC 802

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ATCC 802
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#Make the media solution as follows:
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#*Cerophyll / Cereal Leaves '''2.5 g'''
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1. Make the media solution as follows:
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#*Distilled H2O '''1.0 L'''
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#**Add the cerophyll to the distilled water in a 2L-flask or beaker.
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Cerophyll / Cereal Leaves 2.5 g
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#**Add a stir bar and cover the opening of the flask with foil.  
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Distilled H2O                 1.0 L
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#**Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10).  
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#**Add 20 mL of distilled H2O to compensate for evaporation.
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a. Add the cerophyll to the distilled water in a flask.
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#**Filter through Whatman #1 filter paper.
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b. Add a stir bar and cover the opening of the flask with foil.  
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#**Add 0.94 g of Na2HPO4.7H2O to the solution.
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c. Put the flask on the hot plate. Turn the “Stir” knob up to 6 and the “Heat” knob up to 10. Boil for 5 minutes (will be small bubbles coming up) .  
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#**Distribute the media into media bottles for autoclaving.
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d. Add 20 mL of distilled H2O to compensate for evaporation.
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#**Autoclave for 15 minutes at 121°C.
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e. Filter through Whatman #1 filter paper using the ceramic funnel in the media cabinet.
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#**Label as “ATCC 802” and store in Media cabinet.
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f. Add 0.94 g of Na2HPO4.7H2O to the solution.
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#24 hours prior to inoculation, add the appropriate bacteria (indicated on “[[Colorless Organisms]]” list) for the organism to the media in the tissue flask for culturing.
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g. Autoclave for 15 minutes at 121°C.
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#Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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h. Label as “ATCC 802” and store in Media cabinet.
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2. 24 hours prior to inoculation, add the appropriate bacteria (indicated on “Tina’s Organism List”) for the organism to the media in the tissue flask for culturing.
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3. Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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Current revision as of 18:48, 21 February 2008

  1. Make the media solution as follows:
    • Cerophyll / Cereal Leaves 2.5 g
    • Distilled H2O 1.0 L
      • Add the cerophyll to the distilled water in a 2L-flask or beaker.
      • Add a stir bar and cover the opening of the flask with foil.
      • Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10).
      • Add 20 mL of distilled H2O to compensate for evaporation.
      • Filter through Whatman #1 filter paper.
      • Add 0.94 g of Na2HPO4.7H2O to the solution.
      • Distribute the media into media bottles for autoclaving.
      • Autoclave for 15 minutes at 121°C.
      • Label as “ATCC 802” and store in Media cabinet.
  2. 24 hours prior to inoculation, add the appropriate bacteria (indicated on “Colorless Organisms” list) for the organism to the media in the tissue flask for culturing.
  3. Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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