Snow Algae
From Protists
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== Background == | == Background == | ||
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== Snow Algae Agar Plates == | == Snow Algae Agar Plates == | ||
| - | *measure out 525mL Snow Algae Media (above) into a [[ | + | *measure out 525mL Snow Algae Media (above) into a [[LIVE]] graduated cylinder |
*add 10.5g of Agar (not agarose!!) to make a 2% weight/volume agar concentration | *add 10.5g of Agar (not agarose!!) to make a 2% weight/volume agar concentration | ||
| - | *distribute the mixture into a [[ | + | *distribute the mixture into a [[LIVE]] media bottle(s) |
| - | *heat the mixture on the [[ | + | *heat the mixture on the [[LIVE]] hot/stir plate to dissolve the agar completely |
*[[Autoclave]] the mixture in the bottles on the liquid cycle for the appropriate time | *[[Autoclave]] the mixture in the bottles on the liquid cycle for the appropriate time | ||
Revision as of 14:58, 16 October 2007
Background
These cultures were originally from Ron Hoham and are currently in the UTEX culture collection. We were storing them and maintaining them for use in a collaboration between Ron Hoham (now retired from Colgate) and Dr. Jim Leebens-Mack (plant systematist at UGA). However, most were contaminated with fungi and/or lost because they were killed when the walk-in broke over the summer of 2007 and/or they were not transferred often enough and the agar dried out.
Snow Algae Media Recipe
Snow Algae Agar Plates
- measure out 525mL Snow Algae Media (above) into a LIVE graduated cylinder
- add 10.5g of Agar (not agarose!!) to make a 2% weight/volume agar concentration
- distribute the mixture into a LIVE media bottle(s)
- heat the mixture on the LIVE hot/stir plate to dissolve the agar completely
- Autoclave the mixture in the bottles on the liquid cycle for the appropriate time
