ATCC 802

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ATCC 802
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== ATCC 802 ==
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1. Make the media solution as follows:
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#Make the media solution as follows:
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#*Cerophyll / Cereal Leaves '''2.5 g'''
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Cerophyll / Cereal Leaves 2.5 g
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#*Distilled H2O '''1.0 L'''
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Distilled H2O                 1.0 L
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#**Add the cerophyll to the distilled water in a flask.
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#**Add a stir bar and cover the opening of the flask with foil.  
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a. Add the cerophyll to the distilled water in a flask.
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#**Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10).  
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b. Add a stir bar and cover the opening of the flask with foil.  
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#**Add 20 mL of distilled H2O to compensate for evaporation.
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c. Put the flask on the hot plate. Turn the “Stir” knob up to 6 and the “Heat” knob up to 10. Boil for 5 minutes (will be small bubbles coming up) .  
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#**Filter through Whatman #1 filter paper.
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d. Add 20 mL of distilled H2O to compensate for evaporation.
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#**Add 0.94 g of Na2HPO4.7H2O to the solution.
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e. Filter through Whatman #1 filter paper using the ceramic funnel in the media cabinet.
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#**Distribute the media into media bottles for autoclaving.
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f. Add 0.94 g of Na2HPO4.7H2O to the solution.
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#**Autoclave for 15 minutes at 121°C.
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g. Autoclave for 15 minutes at 121°C.
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#**Label as “ATCC 802” and store in Media cabinet.
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h. Label as “ATCC 802” and store in Media cabinet.
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#24 hours prior to inoculation, add the appropriate bacteria (indicated on “Tina’s Organism List”) for the organism to the media in the tissue flask for culturing.
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2. 24 hours prior to inoculation, add the appropriate bacteria (indicated on “Tina’s Organism List”) for the organism to the media in the tissue flask for culturing.
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#Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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3. Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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Revision as of 18:35, 18 September 2007

ATCC 802

  1. Make the media solution as follows:
    • Cerophyll / Cereal Leaves 2.5 g
    • Distilled H2O 1.0 L
      • Add the cerophyll to the distilled water in a flask.
      • Add a stir bar and cover the opening of the flask with foil.
      • Boil for 5 minutes on the hot plate (“Stir” knob at 6; “Heat” knob at 10).
      • Add 20 mL of distilled H2O to compensate for evaporation.
      • Filter through Whatman #1 filter paper.
      • Add 0.94 g of Na2HPO4.7H2O to the solution.
      • Distribute the media into media bottles for autoclaving.
      • Autoclave for 15 minutes at 121°C.
      • Label as “ATCC 802” and store in Media cabinet.
  2. 24 hours prior to inoculation, add the appropriate bacteria (indicated on “Tina’s Organism List”) for the organism to the media in the tissue flask for culturing.
  3. Incubate the tissue flask in the 33.5°C incubator (middle shelf) overnight. The media is now ready to be inoculated with the protist.
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