View source

== Background ==

These cultures were originally from Ron Hoham and are currently in the UTEX culture collection.  We were storing them and maintaining them for use in a collaboration between Ron Hoham (now retired from Colgate) and Dr. Jim Leebens-Mack (plant systematist at UGA).  However, most were contaminated with fungi and/or lost because they were killed when the walk-in broke over the summer of 2007 and/or they were not transferred often enough and the agar dried out.




== Snow Algae Media Recipe ==





== Snow Algae Agar Plates ==

*measure out 525mL Snow Algae Media (above) into a [[live]] graduated cylinder
*add 10.5g of Agar (not agarose!!) to make a 2% weight/volume agar concentration
*distribute the mixture into a [[live]] media bottle(s)
*heat the mixture on the [[live]] hot/stir plate to dissolve the agar completely
*[[Autoclave]] the mixture in the bottles on the liquid cycle for the appropriate time